PMID- 14641119
OWN - NLM
STAT- MEDLINE
DCOM- 20040324
LR  - 20191210
IS  - 0105-1873 (Print)
IS  - 0105-1873 (Linking)
VI  - 49
IP  - 1
DP  - 2003 Jul
TI  - Utility of historical vehicle-control data in the interpretation of the local 
      lymph node assay.
PG  - 37-41
AB  - The accepted approach to the interpretation of local lymph node assay (LLNA) data 
      requires comparison of responses in the test groups with background activity 
      found in concurrent vehicle-treated controls. However, of established value in 
      the interpretation of toxicity test data is the use of historical control values 
      that provide one criterion against which to judge the integrity of individual 
      experiments. Specifically, the availability of robust and relevant historical 
      control data permits examination of whether, in any individual experiment, 
      control values fall within the expected range. With the most commonly used 
      vehicle employed in the LLNA, acetone/olive oil (4 : 1) (v/v), the mean values, 
      standard deviations and normal ranges are increasingly well established for a 
      given laboratory, although there is some variation between laboratories, 
      particularly with regard to expected ranges. Against this background, it is 
      possible to identify (and, if appropriate, eliminate) a concurrent 
      vehicle-control value that falls well outside the expected range. To explore 
      critically the potential merits of this approach, one specific example is 
      examined in detail.
FAU - Basketter, D A
AU  - Basketter DA
AD  - SEAC, Unilever Colworth, Sharnbrook, Bedford, UK. david.basketter@unilever.com
FAU - Gilmour, N J
AU  - Gilmour NJ
FAU - Briggs, D
AU  - Briggs D
FAU - Ullmann, L G
AU  - Ullmann LG
FAU - Gerberick, G F
AU  - Gerberick GF
FAU - Ryan, C A
AU  - Ryan CA
FAU - Dearman, R J
AU  - Dearman RJ
FAU - Kimber, I
AU  - Kimber I
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Multicenter Study
PL  - England
TA  - Contact Dermatitis
JT  - Contact dermatitis
JID - 7604950
RN  - 0 (Pharmaceutical Vehicles)
RN  - 0 (Pyruvates)
RN  - 3KJM65G5XL (methyl pyruvate)
SB  - IM
MH  - Animals
MH  - Cytotoxicity Tests, Immunologic/*standards
MH  - Dermatitis, Allergic Contact/*immunology/prevention & control
MH  - Europe
MH  - Lymph Nodes/*drug effects
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Pharmaceutical Vehicles/*standards
MH  - Pyruvates/toxicity
MH  - Quality Control
MH  - Reference Standards
MH  - United States
EDAT- 2003/12/04 05:00
MHDA- 2004/03/25 05:00
CRDT- 2003/12/04 05:00
PHST- 2003/12/04 05:00 [pubmed]
PHST- 2004/03/25 05:00 [medline]
PHST- 2003/12/04 05:00 [entrez]
AID - 162 [pii]
AID - 10.1111/j.0105-1873.2003.00162.x [doi]
PST - ppublish
SO  - Contact Dermatitis. 2003 Jul;49(1):37-41. doi: 10.1111/j.0105-1873.2003.00162.x.