PMID- 14642048
OWN - NLM
STAT- MEDLINE
DCOM- 20040621
LR  - 20161018
IS  - 0892-7790 (Print)
IS  - 0892-7790 (Linking)
VI  - 17
IP  - 9
DP  - 2003 Nov
TI  - Evaluation of heat shock protein 70 expression in renal parenchyma subjected to 
      shockwave lithotripsy.
PG  - 805-8
AB  - PURPOSE: In this experimental study in a rabbit model, renal parenchymal heat 
      shock protein 70 (hsp70) levels were assessed in an attempt to evaluate the 
      traumatic effects of high-energy shockwaves (HESW), which have been found to 
      induce transient ischemia during the procedure. MATERIALS AND METHODS: Eighteen 
      white New Zealand rabbits, each weighing 3 to 5 kg, were included in the study. 
      The animals were divided into three groups, and various numbers of shockwaves 
      (1000, 1500, or 2000) were applied to the same kidney of all animals under 
      fluoroscopic localization with a Stonelith V5 lithotripter. Untreated 
      contralateral kidneys were evaluated as controls. Following HESW application, the 
      treated and untreated kidneys were removed surgically after 24 hours or 7 days. 
      Tissue hsp70 levels were assessed by an immunohistochemistry method. RESULTS: 
      During early follow-up (24 hours), both treated and untreated kidneys 
      demonstrated moderate to severe hsp70 positivity. The number of positive tubules 
      increased as the number of shockwaves increased, and positivity became more 
      evident, possibly because of a higher degree of tissue damage. Contralateral 
      kidneys demonstrated a limited degree of hsp70 positivity, although it was not as 
      evident as in the treated kidneys. Assessment of tissue hsp70 levels during late 
      follow-up (7 days) demonstrated moderate or limited degrees of positivity in the 
      treated kidneys. Limited or no positivity could be demonstrated in the untreated 
      kidneys during this period. CONCLUSIONS: Taking the known traumatic effects of 
      HESW and the results of this study into account, the increasing positivity of 
      hsp70 in parallel with the increasing number of shockwaves led us to think about 
      a possible limited degree of ischemia induced by this procedure, as the traumatic 
      effects of HESW were pronounced, as judged by tissue hsp70 positivity.
FAU - Sarica, Kemal
AU  - Sarica K
AD  - Department of Urology, Sahinbey Medical Center, University of Gaziantep, Medical 
      School, Gaziantep, Turkey. Kemalsarica@superonline.com
FAU - Sari, Ibrahim
AU  - Sari I
FAU - Erbagci, Ahmet
AU  - Erbagci A
FAU - Yagci, Faruk
AU  - Yagci F
FAU - Yurtseven, Cihanser
AU  - Yurtseven C
FAU - Karakok, Metin
AU  - Karakok M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Endourol
JT  - Journal of endourology
JID - 8807503
RN  - 0 (HSP70 Heat-Shock Proteins)
SB  - IM
MH  - Animals
MH  - HSP70 Heat-Shock Proteins/*biosynthesis
MH  - *Lithotripsy
MH  - Rabbits
EDAT- 2003/12/04 05:00
MHDA- 2004/06/23 05:00
CRDT- 2003/12/04 05:00
PHST- 2003/12/04 05:00 [pubmed]
PHST- 2004/06/23 05:00 [medline]
PHST- 2003/12/04 05:00 [entrez]
AID - 10.1089/089277903770802425 [doi]
PST - ppublish
SO  - J Endourol. 2003 Nov;17(9):805-8. doi: 10.1089/089277903770802425.