PMID- 14642723 OWN - NLM STAT- MEDLINE DCOM- 20040204 LR - 20190901 IS - 0379-0738 (Print) IS - 0379-0738 (Linking) VI - 138 IP - 1-3 DP - 2003 Dec 17 TI - Morphological and chemical analysis of magic mushrooms in Japan. PG - 85-90 AB - Morphological and toxicological analyses were performed on hallucinogenic mushrooms that are currently circulated in Japan. Scanning electron microscope (SEM) indicated a three-dimensional microstructures in the mushrooms. The complementary use of SEM with an optical microscope was effective for observing characteristic tissues, such as basidiomycetes, spores, cystidia and basidia. Hallucinogenic alkaloids were extracted with methanol and determined by high performance liquid chromatography (HPLC) with a UV detector set at 220 nm. The psilocin/psilocybin contents in Psilocybe cubensis were in the range of 0.14-0.42%/0.37-1.30% in the whole mushroom (0.17-0.78%/0.44-1.35% in the cap and 0.09-0.30%/0.05-1.27% in the stem), respectively. The hallucinogenic alkaloids in Copelandia were 0.43-0.76%/0.08-0.22% in the whole mushroom (0.64-0.74%/0.02-0.22% in the cap and 0.31-0.78%/0.01-0.39% in the stem). It thus appears that P. cubensis is psilocybin-rich, whereas Copelandia is psilocin-rich. FAU - Tsujikawa, Kenji AU - Tsujikawa K AD - National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan. tujikawa@nrips.go.jp FAU - Kanamori, Tatsuyuki AU - Kanamori T FAU - Iwata, Yuko AU - Iwata Y FAU - Ohmae, Yoshihito AU - Ohmae Y FAU - Sugita, Ritsuko AU - Sugita R FAU - Inoue, Hiroyuki AU - Inoue H FAU - Kishi, Tohru AU - Kishi T LA - eng PT - Journal Article PL - Ireland TA - Forensic Sci Int JT - Forensic science international JID - 7902034 RN - 0 (Alkaloids) RN - 0 (Hallucinogens) RN - 0 (Solvents) RN - 2RV7212BP0 (Psilocybin) RN - CMS88KUW0G (psilocin) RN - Y4S76JWI15 (Methanol) SB - IM MH - Agaricales/*chemistry/*ultrastructure MH - Alkaloids/analysis MH - Chromatography, High Pressure Liquid MH - Forensic Medicine MH - Hallucinogens/*chemistry MH - Japan MH - Methanol MH - Microscopy, Electron, Scanning MH - Plant Stems/chemistry MH - Psilocybin/*analogs & derivatives/chemistry MH - Solvents MH - Spores, Fungal/ultrastructure EDAT- 2003/12/04 05:00 MHDA- 2004/02/05 05:00 CRDT- 2003/12/04 05:00 PHST- 2003/12/04 05:00 [pubmed] PHST- 2004/02/05 05:00 [medline] PHST- 2003/12/04 05:00 [entrez] AID - S0379073803003785 [pii] AID - 10.1016/j.forsciint.2003.08.009 [doi] PST - ppublish SO - Forensic Sci Int. 2003 Dec 17;138(1-3):85-90. doi: 10.1016/j.forsciint.2003.08.009.