PMID- 14645241
OWN - NLM
STAT- MEDLINE
DCOM- 20040430
LR  - 20210206
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 279
IP  - 8
DP  - 2004 Feb 20
TI  - Interactions between activating signal cointegrator-2 and the tumor suppressor 
      retinoblastoma in androgen receptor transactivation.
PG  - 7131-5
AB  - Activating signal cointegrator-2 (ASC-2), a cancer-amplified transcription 
      coactivator of nuclear receptors and numerous other transcription factors, was 
      previously shown to contain two LXXLL motifs, each of which interacts with a 
      distinct set of nuclear receptors. In this work, we showed that ASC-2 has an 
      indirect, separate binding site for androgen receptor (AR). Interestingly, this 
      region overlapped with the direct interaction interfaces with the tumor 
      suppressor retinoblastoma (Rb). Although ASC-2 alone stimulated AR 
      transactivation in cotransfections of HeLa cells, ectopic expression of Rb 
      effected ASC-2 to act as a transcription coactivator of AR in Rb-null Saos2 
      cells. These results, along with the previous report in which AR was shown to 
      directly interact with Rb (Yeh, S., Miyamoto, H., Nishimura, K., Kang, H., 
      Ludlow, J., Hsiao, P., Wang, C., Su, C., and Chang C. (1998) Biochem. Biophys. 
      Res. Commun. 248, 361-367), suggest that the AR-ASC-2 interactions in vivo may 
      involve Rb. Thus, ASC-2 appears to contain at least three distinct nuclear 
      receptor interaction domains.
FAU - Goo, Young-Hwa
AU  - Goo YH
AD  - Division of Diabetes, Endocrinology & Metabolism, Department of Medicine, Baylor 
      College of Medicine, Houston, Texas 77030, USA.
FAU - Na, Soon-Young
AU  - Na SY
FAU - Zhang, Hao
AU  - Zhang H
FAU - Xu, Jianming
AU  - Xu J
FAU - Hong, SunHwa
AU  - Hong S
FAU - Cheong, JaeHun
AU  - Cheong J
FAU - Lee, Soo-Kyung
AU  - Lee SK
FAU - Lee, Jae Woon
AU  - Lee JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20031125
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Chromatin)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Luminescent Proteins)
RN  - 0 (NCOA6 protein, human)
RN  - 0 (Nuclear Receptor Coactivators)
RN  - 0 (Receptors, Androgen)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Retinoblastoma Protein)
RN  - 0 (Transcription Factors)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Amino Acid Motifs
MH  - Animals
MH  - Chromatin/metabolism
MH  - Fibroblasts/metabolism
MH  - Glutathione Transferase/metabolism
MH  - Green Fluorescent Proteins
MH  - HeLa Cells
MH  - Humans
MH  - Immunohistochemistry
MH  - *Intracellular Signaling Peptides and Proteins
MH  - Luminescent Proteins/metabolism
MH  - Nuclear Receptor Coactivators
MH  - Plasmids/metabolism
MH  - Precipitin Tests
MH  - Protein Binding
MH  - Protein Structure, Tertiary
MH  - Receptors, Androgen/genetics/*metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Retinoblastoma Protein/*metabolism
MH  - Signal Transduction
MH  - Transcription Factors/*metabolism
MH  - *Transcriptional Activation
EDAT- 2003/12/03 05:00
MHDA- 2004/05/01 05:00
CRDT- 2003/12/03 05:00
PHST- 2003/12/03 05:00 [pubmed]
PHST- 2004/05/01 05:00 [medline]
PHST- 2003/12/03 05:00 [entrez]
AID - S0021-9258(18)44677-7 [pii]
AID - 10.1074/jbc.M312563200 [doi]
PST - ppublish
SO  - J Biol Chem. 2004 Feb 20;279(8):7131-5. doi: 10.1074/jbc.M312563200. Epub 2003 
      Nov 25.