PMID- 14646506
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20031223
LR  - 20191108
IS  - 1360-8185 (Print)
IS  - 1360-8185 (Linking)
VI  - 3
IP  - 2
DP  - 1998 Mar
TI  - cDNA cloning of human DNase gamma: chromosomal localization of its gene and 
      enzymatic properties of recombinant protein.
PG  - 89-95
AB  - We report here on the nucleotide sequence of the cDNA encoding human DNase gamma, 
      which is a candidate for an apoptotic endonuclease. The cDNA clone isolated from 
      a human spleen cDNA library is composed of a 918 bp open reading frame encoding a 
      305 amino acid precursor protein for DNase gamma. Northern blot analysis reveals 
      that the expression of a single transcript of 1.5 kb DNase gamma mRNA is detected 
      in the spleen and liver. The chromosomal localization of DNase gamma gene is 
      mapped to chromosome 3 at region p21.1-p14.2 by fluorescence in situ 
      hybridization (FISH). Characterization of thioredoxin-DNase gamma fusion protein 
      (Trx-hDNase gamma) shows that the recombinant protein has a Ca(2+)/Mg(2+)- or 
      Mn(2+)-dependent endonuclease activity that cleaves chromatin DNA to nucleosomal 
      units. The optimum pH is around 7.2. Zn(2+) and aurintricarboxylic acid (ATA) 
      inhibits the activity in dose-dependent manners. These properties are identical 
      to those of purified DNase gamma.
FAU - Shiokawa, D
AU  - Shiokawa D
AD  - Department of Biochemistry, Faculty of Pharmaceutical Sciences, Science 
      University of Tokyo, Shinjuku-ku, Tokyo, Japan.
FAU - Hirai, M
AU  - Hirai M
FAU - Tanuma, S
AU  - Tanuma S
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Apoptosis
JT  - Apoptosis : an international journal on programmed cell death
JID - 9712129
EDAT- 2003/12/04 05:00
MHDA- 2003/12/04 05:01
CRDT- 2003/12/04 05:00
PHST- 2003/12/04 05:00 [pubmed]
PHST- 2003/12/04 05:01 [medline]
PHST- 2003/12/04 05:00 [entrez]
AID - 282252 [pii]
AID - 10.1023/a:1009692807692 [doi]
PST - ppublish
SO  - Apoptosis. 1998 Mar;3(2):89-95. doi: 10.1023/a:1009692807692.