PMID- 14646597
OWN - NLM
STAT- MEDLINE
DCOM- 20040826
LR  - 20081121
IS  - 1226-3613 (Print)
IS  - 1226-3613 (Linking)
VI  - 35
IP  - 5
DP  - 2003 Oct 31
TI  - NF-kappaB is involved in the TNF-alpha induced inhibition of the differentiation 
      of 3T3-L1 cells by reducing PPARgamma expression.
PG  - 431-7
AB  - TNF-alpha, a trimeric cytokine, was known to inhibit differention of 
      preadipocytes to adipocytes. In the present study, we investigated signal 
      mediators working downstream of TNF-alpha using murine 3T3-L1 cells. TNF-alpha 
      induced activation of both c-jun NH(2)-terminal kinase (JNK) and nuclear 
      transcription factor-kappaB (NF-kappaB) in 3T3-L1 cells. Blockage of these two 
      mediators activities by specific inhibitors, SP600125 and Ad-IkappaBalpha-SR 
      restored adipogenesis differentiation suggesting their involvement in the 
      inhibited differentiation of 3T3-L1 cells by TNF-alpha. Consistent with previous 
      studies, peroxisome proliferator-activated receptor gamma (PPARgamma) a key 
      transcriptional regulator was remarkably reduced by TNF-alpha treatment. Compared 
      with adipogenesis, however, SP600125, a chemical JNK inhibitor hardly relieved 
      TNF-alpha effect on PPARgamma expression whereas S32A/S36A mutant of IkappaBalpha 
      considerably recovered PPARgamma expression, indicating that two signal mediators 
      exploit separable main routes to achieve reduced adipogenesis. These results 
      suggest that inhibition of 3T3-L1 cells differentiation by TNF-alpha is partly 
      implemented through NF-kappaB and one of its downstream effectors be PPARgamma.
FAU - Chae, Gi-Nam
AU  - Chae GN
AD  - Department of Biochemistry, College of Medicine, Dankook University, San 29 
      Anseo-dong, Cheonan 330-714, Korea.
FAU - Kwak, Sahng-June
AU  - Kwak SJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Mol Med
JT  - Experimental & molecular medicine
JID - 9607880
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Transcription Factors)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/cytology/drug effects
MH  - Animals
MH  - Cell Differentiation/*drug effects
MH  - Gene Expression Regulation/*drug effects
MH  - Mice
MH  - NF-kappa B/*metabolism
MH  - Promoter Regions, Genetic/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, Cytoplasmic and Nuclear/*genetics
MH  - Transcription Factors/*genetics
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2003/12/04 05:00
MHDA- 2004/08/27 05:00
CRDT- 2003/12/04 05:00
PHST- 2003/12/04 05:00 [pubmed]
PHST- 2004/08/27 05:00 [medline]
PHST- 2003/12/04 05:00 [entrez]
AID - 2003103113 [pii]
AID - 10.1038/emm.2003.56 [doi]
PST - ppublish
SO  - Exp Mol Med. 2003 Oct 31;35(5):431-7. doi: 10.1038/emm.2003.56.