PMID- 14647002
OWN - NLM
STAT- MEDLINE
DCOM- 20040624
LR  - 20131121
IS  - 0172-780X (Print)
IS  - 0172-780X (Linking)
VI  - 24
IP  - 5
DP  - 2003 Oct
TI  - Melatonin does not affect p21 expression in rat thyroid follicular cells.
PG  - 310-3
AB  - OBJECTIVES: The cell-cycle (cc) is, in part, controlled by cyclin-dependent 
      kinases (CDKs), which are activated after binding cyclins (positive regulators of 
      cc). CDK inhibitors (CDKIs), such as p15, p16, p21, and p27, inhibit cyclin-CDK 
      complex activity, and function as negative cell cycle regulators, blocking 
      cell-cycle progression. The aim of our study was to examine the influence of 
      late-afternoon (1600-1800) subcutaneous (s.c.) melatonin (Mel) injections, 
      administered for 2 weeks, on the p21 expression in male Wistar rat thyroid 
      follicular cells (TFC). METHODS: In order to detect the anti-p21 antibodies, the 
      DAKO LSAB2 Rat Kit/HRP (DAKO, USA) and DAKO Liquid DAB Substrate-Chromogen System 
      (DAKO, USA) were employed. RESULTS: No statistically significant changes of 
      p21(LI) (p21 labelling index) values were observed in particular groups of the 
      rats receiving Mel injections, in comparison to the controls. The statistical 
      difference of p21(LI) values (p<0.03) was only observed between groups of rats, 
      receiving Mel injections in the dose of 25 microg/daily and 100 microg/daily. 
      CONCLUSION: Melatonin - in the applied doses and in the experimental conditions 
      without any factors revealing anti-DNA activity - did not change, in any 
      statistical way, p21 expression in rat thyroid follicular cells, as compared to 
      the control group.
FAU - Gesing, Adam
AU  - Gesing A
AD  - Department of Thyroidology, Institute of Endocrinology, Medical University of 
      Lodz, Dr Sterling St 5, 91-425 Lodz, Poland.
FAU - Jagiela, Joanna
AU  - Jagiela J
FAU - Lewinski, Andrzej
AU  - Lewinski A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Sweden
TA  - Neuro Endocrinol Lett
JT  - Neuro endocrinology letters
JID - 8008373
RN  - 0 (Antioxidants)
RN  - 0 (Cdkn1a protein, rat)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (Cyclins)
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Circadian Rhythm
MH  - Cyclin-Dependent Kinase Inhibitor p21
MH  - Cyclins/*metabolism
MH  - Male
MH  - Melatonin/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Thyroid Gland/cytology/*drug effects/*metabolism
EDAT- 2003/12/04 05:00
MHDA- 2004/06/25 05:00
CRDT- 2003/12/04 05:00
PHST- 2003/01/02 00:00 [received]
PHST- 2003/01/21 00:00 [accepted]
PHST- 2003/12/04 05:00 [pubmed]
PHST- 2004/06/25 05:00 [medline]
PHST- 2003/12/04 05:00 [entrez]
AID - NEL240503A02 [pii]
PST - ppublish
SO  - Neuro Endocrinol Lett. 2003 Oct;24(5):310-3.