PMID- 1464735
OWN - NLM
STAT- MEDLINE
DCOM- 19930115
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 52
IP  - 6
DP  - 1992 Dec
TI  - Tumor necrosis factor-alpha decreases neutrophil chemotaxis to 
      N-formyl-1-methionyl-1-leucyl-1-phenylalanine: analysis of single cell movement.
PG  - 630-6
AB  - Tumor necrosis factor-alpha (TNF-alpha), a cytokine produced by mononuclear cells 
      in response to endotoxin, inhibits neutrophil chemotaxis. We analyzed the effects 
      of TNF-alpha on the orientation and movement of individual neutrophils in a 
      chemoattractant gradient. Neutrophils, treated or untreated with TNF-alpha, were 
      observed migrating in a gradient of the chemotactic peptide 
      N-formyl-1-methionyl-1-leucyl-1-phenylalanine (fMLP) on a specially constructed 
      chamber (Zigmond bridge). The movement of these cells was videotaped, digitized, 
      and then tracked using a newly designed computer algorithm. The data obtained 
      from this algorithm were then utilized to calculate distance traveled, speed and 
      ability to polarize and migrate in a directed manner for each individual cell. 
      TNF-alpha-treated cells behaved like cells not exposed to fMLP in that they 
      failed to orient in a chemotactic gradient and moved in a manner similar to 
      randomly migrating cells. This study provides unique observations of the effect 
      of TNF-alpha on multiple parameters of PMN migration.
FAU - Vollmer, K L
AU  - Vollmer KL
AD  - Division of Infectious Diseases, University of Virginia Health Sciences Center, 
      Charlottesville.
FAU - Alberts, J S
AU  - Alberts JS
FAU - Carper, H T
AU  - Carper HT
FAU - Mandell, G L
AU  - Mandell GL
LA  - eng
GR  - R-37-AI-09504/AI/NIAID NIH HHS/United States
GR  - T32-AI-07046/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
SB  - IM
MH  - Algorithms
MH  - Chemotaxis, Leukocyte/*drug effects
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - In Vitro Techniques
MH  - Kinetics
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Neutrophils/drug effects/*physiology
MH  - Recombinant Proteins/pharmacology
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Videotape Recording/instrumentation/methods
EDAT- 1992/12/01 00:00
MHDA- 1992/12/01 00:01
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 1992/12/01 00:01 [medline]
PHST- 1992/12/01 00:00 [entrez]
AID - 10.1002/jlb.52.6.630 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1992 Dec;52(6):630-6. doi: 10.1002/jlb.52.6.630.