PMID- 14648664
OWN - NLM
STAT- MEDLINE
DCOM- 20040227
LR  - 20061115
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 201
IP  - 4
DP  - 2003 Dec
TI  - High-throughput tissue microarray analysis of 11q13 gene amplification (CCND1, 
      FGF3, FGF4, EMS1) in urinary bladder cancer.
PG  - 603-8
AB  - Gene amplification is a common mechanism for oncogene overexpression. High-level 
      amplifications at 11q13 have been repeatedly found in bladder cancer by 
      comparative genomic hybridization (CGH) and other techniques. Putative candidate 
      oncogenes located in this region are CCND1 (PRAD1, bcl-1), EMS1, FGF3 (Int-2), 
      and FGF4 (hst1, hstf1). To evaluate the involvement of these genes in bladder 
      cancer, a tissue microarray (TMA) containing 2317 samples was screened by 
      fluorescence in situ hybridization (FISH). The frequency of gains and 
      amplifications of all genes increased significantly from stage pTa to pT1-4 and 
      from low to high grade. In addition, amplification was associated with patient 
      survival and progression of pT1 tumours. Among 123 tumours with amplifications, 
      68.3% showed amplification of all four genes; 19.5% amplification of CCND1, FGF4, 
      and FGF3; and 0.8% co-amplification of FGF4, FGF3, and EMS1. Amplification of 
      CCND1 alone was found in 9% of the tumours, while EMS1 alone was amplified in 
      1.6% and FGF4 in 0.8%. Overall, the amplification frequency decreased with 
      increasing genomic distance from CCND1, suggesting that, among the genes 
      examined, CCND1 is the major target gene in the 11q13 amplicon in bladder cancer.
CI  - Copyright 2003 John Wiley & Sons, Ltd.
FAU - Zaharieva, Boriana M
AU  - Zaharieva BM
AD  - Department of Medical Genetics, Medical University, Zdrave 2, 1431 Sofia, 
      Bulgaria.
FAU - Simon, Ronald
AU  - Simon R
FAU - Diener, Pierre-Andre
AU  - Diener PA
FAU - Ackermann, Daniel
AU  - Ackermann D
FAU - Maurer, Robert
AU  - Maurer R
FAU - Alund, Goran
AU  - Alund G
FAU - Knonagel, Hartmut
AU  - Knonagel H
FAU - Rist, Marcus
AU  - Rist M
FAU - Wilber, Kim
AU  - Wilber K
FAU - Hering, Franz
AU  - Hering F
FAU - Schonenberger, Andreas
AU  - Schonenberger A
FAU - Flury, Renata
AU  - Flury R
FAU - Jager, Peter
AU  - Jager P
FAU - Fehr, Jean Luc
AU  - Fehr JL
FAU - Mihatsch, Michael J
AU  - Mihatsch MJ
FAU - Gasser, Thomas
AU  - Gasser T
FAU - Sauter, Guido
AU  - Sauter G
FAU - Toncheva, Draga I
AU  - Toncheva DI
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (CTTN protein, human)
RN  - 0 (Cortactin)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (FGF3 protein, human)
RN  - 0 (FGF4 protein, human)
RN  - 0 (Fibroblast Growth Factor 3)
RN  - 0 (Fibroblast Growth Factor 4)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 136601-57-5 (Cyclin D1)
RN  - 62031-54-3 (Fibroblast Growth Factors)
SB  - IM
MH  - Adenocarcinoma/genetics
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Small Cell/genetics
MH  - Carcinoma, Squamous Cell/genetics
MH  - Carcinoma, Transitional Cell/genetics
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Cortactin
MH  - Cyclin D1/genetics
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Fibroblast Growth Factor 3
MH  - Fibroblast Growth Factor 4
MH  - Fibroblast Growth Factors/genetics
MH  - Gene Amplification/*genetics
MH  - Genes, bcl-1/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Microfilament Proteins/genetics
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Oligonucleotide Array Sequence Analysis/*methods
MH  - Oncogenes/*genetics
MH  - Phenotype
MH  - Prognosis
MH  - Proto-Oncogene Proteins/*genetics
MH  - Urinary Bladder Neoplasms/*genetics
EDAT- 2003/12/04 05:00
MHDA- 2004/02/28 05:00
CRDT- 2003/12/04 05:00
PHST- 2003/12/04 05:00 [pubmed]
PHST- 2004/02/28 05:00 [medline]
PHST- 2003/12/04 05:00 [entrez]
AID - 10.1002/path.1481 [doi]
PST - ppublish
SO  - J Pathol. 2003 Dec;201(4):603-8. doi: 10.1002/path.1481.