PMID- 14648666
OWN - NLM
STAT- MEDLINE
DCOM- 20040227
LR  - 20151119
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 201
IP  - 4
DP  - 2003 Dec
TI  - Productive infection of dendritic cells by simian immunodeficiency virus in 
      macaque intestinal tissues.
PG  - 616-28
AB  - Dendritic cells (DCs) are potent antigen-presenting cells that likely play 
      multiple roles in human immunodeficiency virus-1 (HIV-1) and simian 
      immunodeficiency virus (SIV) pathogenesis. This paper describes the effects of 
      pathogenic SIV infection on the networks of DCs in rhesus macaque (Macaca 
      mulatta) intestinal tissues. Intestinal tissues were obtained from macaques at 
      different stages of disease following infection with the pathogenic SIV/DeltaB670 
      isolate. The patterns and levels of expression of SIV and DC-associated mRNAs 
      were examined and quantitated directly in intestinal tissue sections. In situ 
      hybridization was performed for SIV, DC-specific ICAM3-grabbing non-integrin 
      (DC-SIGN), DC-specific lysosome-associated membrane glycoprotein (DC-LAMP), 
      DC-specific C-type lectin 1 (DECTIN-1), CC chemokine receptor 6 (CCR6), CCR7, and 
      macrophage inflammatory protein 3alpha (MIP-3alpha/CCL20) mRNAs and quantitative 
      image analysis was performed to measure mRNA expression levels. To identify the 
      cell types productively infected by SIV, simultaneous in situ hybridization and 
      immunohistochemical staining were performed. The DC networks in macaque 
      intestinal tissues were found to be extensive and although they generally 
      remained intact during the course of SIV infection, there were alterations in the 
      expression of markers for immature DCs. One alteration was an increase in the 
      expression in intestinal submucosa of DC-SIGN, a molecule that binds to HIV-1/SIV 
      and increases its infectivity. Concomitant with this increase, it was found that 
      during AIDS, the population of productively infected cells included DCs, based on 
      co-expression of DC-SIGN and DECTIN-1 mRNAs. These data indicate that SIV 
      infection affects subpopulations of macaque intestinal DCs, including productive 
      infection of DC-SIGN+ DCs, the consequences of which are likely to be ongoing 
      viral propagation and decreased immunostimulatory function.
CI  - Copyright 2003 John Wiley & Sons, Ltd.
FAU - Choi, Yang Kyu
AU  - Choi YK
AD  - Department of Infectious Diseases and Microbiology, University of Pittsburgh, 
      Pittsburgh, PA 15261, USA.
FAU - Whelton, Kelly M
AU  - Whelton KM
FAU - Mlechick, Brandy
AU  - Mlechick B
FAU - Murphey-Corb, Michael A
AU  - Murphey-Corb MA
FAU - Reinhart, Todd A
AU  - Reinhart TA
LA  - eng
GR  - HL62056/HL/NHLBI NIH HHS/United States
GR  - MH61205/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (Biomarkers)
RN  - 0 (CD68 antigen, human)
RN  - 0 (Chemokines)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Acquired Immunodeficiency Syndrome/pathology
MH  - Animals
MH  - Antigens, CD/analysis
MH  - Antigens, Differentiation, Myelomonocytic/analysis
MH  - Biomarkers/analysis
MH  - Chemokines/analysis
MH  - Colon/pathology/virology
MH  - Dendritic Cells/*virology
MH  - Epithelium/pathology/virology
MH  - HLA-DR Antigens/analysis
MH  - Immunohistochemistry/methods
MH  - In Situ Hybridization/methods
MH  - Intestinal Diseases/*pathology
MH  - Intestinal Mucosa/pathology/virology
MH  - Jejunum/pathology/virology
MH  - Macaca mulatta
MH  - Muscle, Smooth/pathology/virology
MH  - Peyer's Patches/pathology/virology
MH  - RNA, Messenger/analysis
MH  - RNA, Viral/analysis
MH  - Receptors, Chemokine/analysis
MH  - Simian Acquired Immunodeficiency Syndrome/*pathology
MH  - Simian Immunodeficiency Virus/*pathogenicity
EDAT- 2003/12/04 05:00
MHDA- 2004/02/28 05:00
CRDT- 2003/12/04 05:00
PHST- 2003/12/04 05:00 [pubmed]
PHST- 2004/02/28 05:00 [medline]
PHST- 2003/12/04 05:00 [entrez]
AID - 10.1002/path.1482 [doi]
PST - ppublish
SO  - J Pathol. 2003 Dec;201(4):616-28. doi: 10.1002/path.1482.