PMID- 14648881
OWN - NLM
STAT- MEDLINE
DCOM- 20040727
LR  - 20101118
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 67
IP  - 1
DP  - 2004 Jan
TI  - Sexing river buffalo (Bubalus bubalis L.), sheep (Ovis aries L.), goat (Capra 
      hircus L.), and cattle spermatozoa by double color FISH using bovine (Bos taurus 
      L.) X- and Y-painting probes.
PG  - 108-15
AB  - River buffalo, sheep, and goat spermatozoa were cross-hybridized using double 
      color fluorescence in situ hybridization (FISH) with bovine Xcen- and 
      Y-chromosome painting probes, prepared by DOP-PCR of 
      laser-microdissected-catapulted chromosomes, to investigate the possibility of 
      using bovine probes for sexing sperm of other members of the family Bovidae. 
      Before sperm analysis, the probes were hybridized on metaphase chromosomes of 
      each species, as control. Frozen-thawed spermatozoa of cattle, river buffalo, 
      sheep, and goat were decondensed in suspension with 5 mM DTT. Sperm samples 
      obtained from three individuals of each species were investigated, more than 
      1,000 spermatozoa were scored in each animal. FISH analysis of more than 12,000 
      sperm revealed high level of sperm with X- or Y-signals in all of the species 
      investigated, indicating FISH efficiency over 99%. Significant interspecific 
      differences were detected in the frequency of aberrant spermatozoa (aneuploid and 
      diploid) between goat (0.393%) and sheep (0.033%) (P < 0.01), goat and cattle 
      (0.096%) (P < 0.5), as well as between river buffalo (0.224%) and sheep (P < 
      0.5). There was no significant difference between river buffalo and cattle. The 
      present study demonstrated that it is possible to use bovine X-Y painting probes 
      for sexing and analyzing sperm of other species of the family, thus facilitating 
      future studies on the incidence of chromosome abnormalities in sperm as well as 
      on sex predetermination of embryos for the livestock industry. Mol. Reprod. Dev. 
      67: 108-115, 2004.
CI  - Copyright 2004 Wiley-Liss, Inc.
FAU - Di Berardino, Dino
AU  - Di Berardino D
AD  - Department of Animal Science and Food Inspection, University of Naples "Federico 
      II," Naples, Italy. diberard@unina.it
FAU - Vozdova, Miluse
AU  - Vozdova M
FAU - Kubickova, Svatava
AU  - Kubickova S
FAU - Cernohorska, Halina
AU  - Cernohorska H
FAU - Coppola, Giuseppe
AU  - Coppola G
FAU - Coppola, Gianfranco
AU  - Coppola G
FAU - Enne, Giuseppe
AU  - Enne G
FAU - Rubes, Jiri
AU  - Rubes J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Animals
MH  - *Buffaloes
MH  - *Cattle
MH  - Fluorescent Dyes
MH  - *Goats
MH  - In Situ Hybridization
MH  - *In Situ Hybridization, Fluorescence
MH  - Lasers
MH  - Male
MH  - Sex Determination Analysis/*veterinary
MH  - *Sheep
MH  - Species Specificity
MH  - *Spermatozoa/cytology
MH  - X Chromosome/genetics/metabolism
MH  - Y Chromosome/genetics/metabolism
EDAT- 2003/12/04 05:00
MHDA- 2004/07/28 05:00
CRDT- 2003/12/04 05:00
PHST- 2003/12/04 05:00 [pubmed]
PHST- 2004/07/28 05:00 [medline]
PHST- 2003/12/04 05:00 [entrez]
AID - 10.1002/mrd.20016 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2004 Jan;67(1):108-15. doi: 10.1002/mrd.20016.