PMID- 14649381
OWN - NLM
STAT- MEDLINE
DCOM- 20040629
LR  - 20240505
IS  - 1058-0468 (Print)
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Linking)
VI  - 20
IP  - 10
DP  - 2003 Oct
TI  - The relationship of pronuclear stage morphology and chromosome status at cleavage 
      stage.
PG  - 413-20
AB  - PURPOSE: Infertile couples undergoing in vitro fertilization-embryo transfer 
      (IVF-ET) program were included to study the relationship of pronuclear stage 
      morphology and chromosome status at cleavage stage. METHODS: Eighteen to 
      twenty-one hours after fertilization, zygotes were checked for pronuclear 
      morphology with modified Scott Z-score system. After embryo transfer on day 3, 
      arrested or non-transferred 2 PN embryos were spread for fluorescence in situ 
      hybridization (FISH) staining of probes to chromosomes 18, X and Y. RESULTS: 
      Ninety-eight embryos were successfully fixed and stained. The chromosome status 
      were recorded in each 2 PN score group: 7 (54%) of 13 embryos in Z2 group, 14 
      (35%) of 40 in Z3 group and 10 (36%) of 28 in Z4 group being normal diploid. Z1 
      group has 12 (71%) of 17 embryos being normal diploid, which is significantly 
      more than Z3 (p = 0.020) and Z4 group (p = 0.033). CONCLUSION: Our results 
      demonstrated a high probability to get normal diploid embryos if good morphology 
      at pronuclear stage was used as selection criteria, especially for Z1 score 
      embryos.
FAU - Chen, Chun-Kai
AU  - Chen CK
AD  - Department of Obstetrics and Gynecology, Lin-Kou Medical Center, Chang Gung 
      Memorial Hospital and Medical College, 5, Fu-Hsing Street, Kwei-Shan, Tao-Yuan 
      333, Taiwan. ckchen@cgmh.org.tw
FAU - Shen, Guan-Yi
AU  - Shen GY
FAU - Horng, Shang-Gwo
AU  - Horng SG
FAU - Wang, Chia-Woei
AU  - Wang CW
FAU - Huang, Hong-Yuan
AU  - Huang HY
FAU - Wang, Hsin-Shin
AU  - Wang HS
FAU - Soong, Yung-Kuei
AU  - Soong YK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
SB  - IM
MH  - Blastomeres/cytology/physiology
MH  - *Chromosomes, Human
MH  - Chromosomes, Human, X
MH  - Chromosomes, Human, Y
MH  - Diploidy
MH  - Embryo Implantation
MH  - *Embryo Transfer
MH  - Embryonic and Fetal Development/*physiology
MH  - Female
MH  - *Fertilization in Vitro
MH  - Humans
MH  - Male
MH  - Oocytes/*cytology/physiology
MH  - *Ovulation Induction
MH  - Patient Selection
MH  - Pregnancy
MH  - Probability
MH  - Zygote/ultrastructure
PMC - PMC3455171
EDAT- 2003/12/03 05:00
MHDA- 2004/06/30 05:00
PMCR- 2004/10/01
CRDT- 2003/12/03 05:00
PHST- 2003/12/03 05:00 [pubmed]
PHST- 2004/06/30 05:00 [medline]
PHST- 2003/12/03 05:00 [entrez]
PHST- 2004/10/01 00:00 [pmc-release]
AID - 474259 [pii]
AID - 10.1023/a:1026232625659 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2003 Oct;20(10):413-20. doi: 10.1023/a:1026232625659.