PMID- 14649462
OWN - NLM
STAT- MEDLINE
DCOM- 20040805
LR  - 20041117
IS  - 1080-2924 (Print)
IS  - 1080-2924 (Linking)
VI  - 9
IP  - 4
DP  - 2003
TI  - Human reticulocytes isolated from peripheral blood: maturation time and 
      hemoglobin synthesis.
PG  - 198-206
AB  - A pure population of young reticulocytes was isolated from the blood of healthy 
      blood donors by an immunomagnetic technique. The young reticulocytes isolated had 
      a larger mean corpuscular volume (MCV) but lower mean corpuscular hemoglobin 
      (MCH) and mean corpuscular hemoglobin concentration (MCHC) than reticulocytes in 
      peripheral blood. The cells were incubated in vitro for 5 days with hemolysis 
      less than 5%. Maturation of reticulocytes was studied with 4 different systems: 
      new methylene blue-stained smears and flow cytometry after auramine O, oxazine 
      750, or thiazole orange staining. The maturation process from reticulocytes to 
      erythrocytes had a half-life of 30 hours estimated by visual counting. 
      Reticulocytes analyzed with flow cytometers had half-lives of 20, 28, and 29 
      hours with Coulter Epics XL, Sysmex R-1000, and Bayer H*3 devices, respectively. 
      Total maturation time was 4 to 5 days. During maturation, the reticulocyte MCV 
      gradually decreased with a concomitant increase in reticulocyte MCHC. The 
      reticulocytes synthesized hemoglobin, and the MCH of the cells increased 
      approximately 7% during the incubation period. Taking into account loss of 
      hemoglobin-containing vesicles during reticulocyte maturation, we estimated that 
      more than 20% of hemoglobin in erythrocytes was synthesized in reticulocytes 
      after release from bone marrow.
FAU - Skadberg, Oyvind
AU  - Skadberg O
AD  - Institute of Clinical Biochemistry, Haukeland University Hospital, University of 
      Bergen, Bergen, Norway. oyvind.skadberg@helse-bergen.no
FAU - Brun, Atle
AU  - Brun A
FAU - Sandberg, Sverre
AU  - Sandberg S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Lab Hematol
JT  - Laboratory hematology : official publication of the International Society for 
      Laboratory Hematology
JID - 9615688
RN  - 0 (Hemoglobins)
RN  - 0 (hemoglobin A, carboxymethylated-)
RN  - 9034-51-9 (Hemoglobin A)
SB  - IM
MH  - Blood Cells
MH  - Cell Differentiation
MH  - Cell Size
MH  - Cells, Cultured
MH  - *Erythropoiesis
MH  - Hemoglobin A/biosynthesis
MH  - Hemoglobins/*biosynthesis
MH  - Humans
MH  - Immunomagnetic Separation
MH  - Kinetics
MH  - Reticulocytes/*cytology/metabolism
EDAT- 2003/12/03 05:00
MHDA- 2004/08/06 05:00
CRDT- 2003/12/03 05:00
PHST- 2003/12/03 05:00 [pubmed]
PHST- 2004/08/06 05:00 [medline]
PHST- 2003/12/03 05:00 [entrez]
PST - ppublish
SO  - Lab Hematol. 2003;9(4):198-206.