PMID- 14649488
OWN - NLM
STAT- MEDLINE
DCOM- 20040706
LR  - 20220309
IS  - 0090-6964 (Print)
IS  - 0090-6964 (Linking)
VI  - 31
IP  - 10
DP  - 2003 Nov
TI  - Regenerative versus reparative healing in tendon: a study of biomechanical and 
      histological properties in fetal sheep.
PG  - 1143-52
AB  - Previous studies have shown fetal tissues heal in a regenerative fashion without 
      scar formation. The objective of this study is to compare the healing properties 
      of adult and fetal tendons. Time-mated pregnant ewes at 80-85 days of gestation 
      were utilized. A partial, midsubstance tenotomy was performed in the lateral 
      extensor fetal tendons, and analogous tenotomies were created in the maternal 
      limbs. One week after injury, the fetal and adult animals were sacrificed, and 
      tendons were histologically and mechanically evaluated. Immunohistochemical 
      staining for transforming growth factor beta isoform 1 (TGF-beta1) was performed. 
      Histologically, a gap with granulation tissue and inflammatory cells was visible 
      in the site of wounding in the adult tendons. In the fetal tendons, no 
      abnormalities were noted in the wound, with reconstitution of collagen 
      architecture. TGF-beta1 expression was low in fetal but upregulated in the adult 
      wounds. No significant differences were found in the biomechanical properties 
      between groups. We identified regenerative healing properties in injured fetal 
      tendon, while adult tendon tissue healed reparatively with scar formation. Fetal 
      tendons demonstrated a limited recovery of mechanical properties after injury 
      that was no better than that of the adult tendons at seven days. A better 
      understanding of the mechanisms of fetal healing may lead to novel therapeutic 
      strategies in the clinical setting.
FAU - Beredjiklian, Pedro K
AU  - Beredjiklian PK
AD  - McKay Orthopaedic Research Laboratory, University of Pennsylvania, Philadelphia, 
      PA, USA. pedro.beredjiklian@uphs.upenn.edu
FAU - Favata, Michele
AU  - Favata M
FAU - Cartmell, Jeffrey S
AU  - Cartmell JS
FAU - Flanagan, Colleen L
AU  - Flanagan CL
FAU - Crombleholme, Timothy M
AU  - Crombleholme TM
FAU - Soslowsky, Louis J
AU  - Soslowsky LJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Ann Biomed Eng
JT  - Annals of biomedical engineering
JID - 0361512
SB  - IM
MH  - *Aging
MH  - Animals
MH  - Elasticity
MH  - Female
MH  - Male
MH  - Regeneration/physiology
MH  - Sheep
MH  - Tendon Injuries/embryology/*pathology/*physiopathology
MH  - Tensile Strength
MH  - Viscosity
MH  - Wound Healing/*physiology
EDAT- 2003/12/03 05:00
MHDA- 2004/07/09 05:00
CRDT- 2003/12/03 05:00
PHST- 2003/12/03 05:00 [pubmed]
PHST- 2004/07/09 05:00 [medline]
PHST- 2003/12/03 05:00 [entrez]
AID - 10.1114/1.1616931 [doi]
PST - ppublish
SO  - Ann Biomed Eng. 2003 Nov;31(10):1143-52. doi: 10.1114/1.1616931.