PMID- 14650181
OWN - NLM
STAT- MEDLINE
DCOM- 20040813
LR  - 20141120
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 32
IP  - 5
DP  - 2003 Sep
TI  - [Application of precision-cut liver slice to the study of the effect of 
      glutathione on cadmium chloride hepatotoxicity].
PG  - 429-31
AB  - In order to evaluate the feasibility of application of precision cut liver 
      slice(PCLS) to the study of the effect of glutathione on cadmium chloride 
      hepatotoxicity, slices were cultured with CdCl2 and GSH. Slices activity and drug 
      metabolic function was estimated by assay of slice viability, NO secretion, and 
      phase I and phase II enzyme activity. Results showed that the slices activity was 
      significantly inhibited by CdCl2. Various phase I and phase II enzymes activity 
      of slices were obviously reduced by CdCl2, but aniline hydroxidase activity was 
      enhanced. GSH could distinctly reversed the change of slices induced by CdCl2. 
      Therefore, it could be concluded that PCLS culture system was sensitive and 
      steady, and was a good model for pharmacological and toxicological investigation 
      of liver in vitro.
FAU - Yang, Zheqiong
AU  - Yang Z
AD  - Department of Pharmacology, Medical College, Wuhan University, Wuhan 430071, 
      China.
FAU - Peng, Renxiu
AU  - Peng R
FAU - Xi, Jinlei
AU  - Xi J
FAU - Wu, Junyu
AU  - Wu J
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - GAN16C9B8O (Glutathione)
RN  - J6K4F9V3BA (Cadmium Chloride)
SB  - IM
MH  - Animals
MH  - Cadmium Chloride/antagonists & inhibitors/*toxicity
MH  - Culture Techniques
MH  - Cytochrome P-450 Enzyme System/metabolism
MH  - Glutathione/*pharmacology
MH  - Glutathione Transferase/metabolism
MH  - Liver/*drug effects/metabolism
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2003/12/03 05:00
MHDA- 2004/08/17 10:00
CRDT- 2003/12/03 05:00
PHST- 2003/12/03 05:00 [pubmed]
PHST- 2004/08/17 10:00 [medline]
PHST- 2003/12/03 05:00 [entrez]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2003 Sep;32(5):429-31.