PMID- 14650434
OWN - NLM
STAT- MEDLINE
DCOM- 20040204
LR  - 20151119
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 23
IP  - 1
DP  - 1996 Jan 1
TI  - Combination of lamin immunocytochemistry and in situ hybridization for the 
      analysis of chromosome copy numbers in tumor cell areas with high nuclear 
      density.
PG  - 1-7
AB  - We describe the application of lamin immunocytochemistry (ICC) and single- or 
      double-target fluorescence in situ hybridization (FISH) on 4 microm thick frozen 
      tissue sections as a method to facilitate scoring of aberrant chromosome copy 
      numbers in colonic tumors. Analysis of FISH signals in colon tissue sections is 
      often hampered by overlap and truncation of epithelial nuclei, due to the density 
      of the epithelial cells. Furthermore, on the basis of nuclear staining it is 
      often difficult to determine whether or not nuclei are overlapping, or adjoining. 
      Therefore, reliable evaluation of (F)ISH signals to screen for genomic changes 
      was until now mainly restricted to isolated nuclei obtained from relatively thick 
      tissue sections. In this study the applicability of lamin ICC, to stain the 
      nuclear periphery and to distinguish individual nuclei, combined with the FISH 
      procedure is explored to solve this problem for colon epithelium. For ICC we 
      applied the alkaline phosphatase (APase)-Fast Red detection method, since the 
      fluorescent precipitate of this reaction resists extensive proteolytic digestion 
      as needed for efficient FISH on tissue sections. Chromosome copy numbers could 
      easily be determined in 4 microm thick frozen tissue sections by combining lamin 
      ICC and FISH. The ratio of the copy numbers of the chromosomes 7 and 17 could be 
      determined in frozen tissue sections after combined lamin ICC and double-target 
      FISH. It is concluded that the combination of lamin ICC and FISH improves 
      chromosome copy number analysis and can be used to investigate genomic changes in 
      different tumor compartments in thin frozen tissue sections.
FAU - Herbergs, J
AU  - Herbergs J
AD  - Department of Pathology, University Hospital Maastricht, The Netherlands.
FAU - Speel, E J
AU  - Speel EJ
FAU - Ramaekers, F C
AU  - Ramaekers FC
FAU - de Bruine, A P
AU  - de Bruine AP
FAU - Arends, J W
AU  - Arends JW
FAU - Hopman, A H
AU  - Hopman AH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (Azo Compounds)
RN  - 0 (Lamins)
RN  - 1658-56-6 (Fast Red S)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase
MH  - *Aneuploidy
MH  - Azo Compounds
MH  - Carcinoma/*genetics/pathology
MH  - Cell Nucleus/*genetics/pathology
MH  - Chromosome Aberrations
MH  - Colonic Neoplasms/*genetics/pathology
MH  - Cytogenetic Analysis/methods
MH  - Humans
MH  - Immunohistochemistry/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lamins/*metabolism
MH  - Microtomy/methods
MH  - Nuclear Envelope/metabolism/ultrastructure
EDAT- 1996/01/01 00:00
MHDA- 2004/02/05 05:00
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 2004/02/05 05:00 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1002/(SICI)1097-0320(19960101)23:1<1::AID-CYTO1>3.0.CO;2-P [doi]
PST - ppublish
SO  - Cytometry. 1996 Jan 1;23(1):1-7. doi: 
      10.1002/(SICI)1097-0320(19960101)23:1<1::AID-CYTO1>3.0.CO;2-P.