PMID- 14654972
OWN - NLM
STAT- MEDLINE
DCOM- 20040316
LR  - 20190816
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 13
IP  - 1
DP  - 2004 Jan
TI  - Increased amplification proximal to the MLL gene in acute myeloid leukemia.
PG  - 63-7
AB  - Amplification of the chromosomal region 11q23 encompassing the MLL gene has been 
      observed in patients with acute myeloid leukemia (AML). Patients with this 
      abnormality often have a poor response to chemotherapy and short survival. We 
      have studied the regions flanking the MLL gene using 8 bacterial artificial 
      chromosome (BAC) probes and dual color fluorescence in situ hybridization (FISH) 
      analysis. Two contigs of BAC probes flanking the MLL gene, were constructed by 
      standard primer walking and BAC end sequencing. For comparison, metaphase 
      chromosome preparations were also hybridized with a commercial MLL specific 
      probe. Metaphase chromosomes were prepared from the bone marrow sample of an 
      80-year old female patient with AML-M1 and the cytogenetic aberration 
      der(11)hsr(11) (q23). FISH analysis on metaphase chromosomes showed amplification 
      on the homogeneously staining region (hsr) and marker chromosomes for both the 
      MLL gene and the BAC probes. Using dual color FISH, probes proximal to MLL showed 
      greater amplification than those distal to MLL, as represented by additional red 
      signals on both metaphase and interphase chromosomes. Ratios of the copy numbers 
      of the BAC probes relative to the chromosome 11 centromere copy number confirmed 
      a higher copy number for probes proximal to MLL. These results suggest that other 
      gene(s) proximal to MLL could be the target gene(s) of amplification in this case 
      and not the MLL gene as previously assumed.
FAU - Lee, Ann Siew-Gek
AU  - Lee AS
AD  - Division of Medical Sciences, National Cancer Centre, Singapore 169610. 
      dmslsg@nccs.com.sg
FAU - Lie, Daniel Khun-Hong
AU  - Lie DK
FAU - Tien, Sim-Leng
AU  - Tien SL
FAU - Rudduck-Sivaswaren, Christina
AU  - Rudduck-Sivaswaren C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (KMT2A protein, human)
RN  - 0 (Transcription Factors)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
SB  - IM
MH  - Acute Disease
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 11
MH  - DNA-Binding Proteins/*genetics
MH  - Female
MH  - *Gene Amplification
MH  - Histone-Lysine N-Methyltransferase
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myeloid/*genetics
MH  - Myeloid-Lymphoid Leukemia Protein
MH  - *Proto-Oncogenes
MH  - *Transcription Factors
EDAT- 2003/12/05 05:00
MHDA- 2004/03/17 05:00
CRDT- 2003/12/05 05:00
PHST- 2003/12/05 05:00 [pubmed]
PHST- 2004/03/17 05:00 [medline]
PHST- 2003/12/05 05:00 [entrez]
PST - ppublish
SO  - Int J Mol Med. 2004 Jan;13(1):63-7.