PMID- 14656147
OWN - NLM
STAT- MEDLINE
DCOM- 20040809
LR  - 20191210
IS  - 8756-7938 (Print)
IS  - 1520-6033 (Linking)
VI  - 19
IP  - 6
DP  - 2003 Nov-Dec
TI  - Application of magnetic agarose support in liquid magnetically stabilized 
      fluidized bed for protein adsorption.
PG  - 1721-7
AB  - A novel magnetic agarose support (MAS) was fabricated for application in a liquid 
      magnetically stabilized fluidized bed (MSFB). It was produced by water-in-oil 
      emulsification method using a mixture of agarose solution and nanometer-sized 
      superparamagnetic Fe(3)O(4) particles as the aqueous phase. The MAS showed good 
      superparamagnetic responsiveness in a magnetic field. A reactive triazine dye, 
      Cibacron blue 3GA (CB), was coupled to the gel to prepare a CB-modified magnetic 
      agarose support (CB-MAS) for protein adsorption. Lysozyme was used as a model 
      protein to test the adsorption equilibrium and kinetic behavior of the CB-MAS. 
      The dependence of bed expansion in the MSFB with a transverse magnetic field on 
      liquid velocity and magnetic field intensity was investigated. Liquid-phase 
      dispersion behavior in the MSFB was examined by measurements of residence time 
      distributions and compared with that obtained in packed and expanded beds. 
      Dynamic lysozyme adsorption in the MSFB was also compared with those in packed 
      and expanded beds. The dynamic binding capacity at 10% breakthrough was estimated 
      at 55.8 mg/mL in the MSFB, higher than that in the expanded bed (31.1 mg/mL) at a 
      liquid velocity of 45 cm/h. The results indicate that the CB-MAS is promising for 
      use in liquid MSFB for protein adsorption.
FAU - Tong, Xiao-Dong
AU  - Tong XD
AD  - Department of Biochemical Engineering, School of Chemical Engineering and 
      Technology, Tianjin University, Tianjin 300072, China.
FAU - Sun, Yan
AU  - Sun Y
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biotechnol Prog
JT  - Biotechnology progress
JID - 8506292
RN  - 0 (Egg Proteins)
RN  - 0 (Proteins)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Adsorption
MH  - Chromatography, Liquid/*methods
MH  - Egg Proteins/isolation & purification
MH  - Electrophoresis, Agar Gel/*methods
MH  - *Magnetics
MH  - Muramidase/*isolation & purification
MH  - Proteins/isolation & purification
EDAT- 2003/12/06 05:00
MHDA- 2004/08/10 05:00
CRDT- 2003/12/06 05:00
PHST- 2003/12/06 05:00 [pubmed]
PHST- 2004/08/10 05:00 [medline]
PHST- 2003/12/06 05:00 [entrez]
AID - 10.1021/bp030028p [doi]
PST - ppublish
SO  - Biotechnol Prog. 2003 Nov-Dec;19(6):1721-7. doi: 10.1021/bp030028p.