PMID- 14656751
OWN - NLM
STAT- MEDLINE
DCOM- 20040226
LR  - 20061115
IS  - 1073-449X (Print)
IS  - 1073-449X (Linking)
VI  - 169
IP  - 4
DP  - 2004 Feb 15
TI  - Regulation of mite allergen-pulsed murine dendritic cells by respiratory 
      syncytial virus.
PG  - 494-8
AB  - Dendritic cells (DCs) are the only antigen-presenting cells that determine T-cell 
      differentiation and play an important role in both allergy and viral infection. 
      Respiratory syncytial virus (RSV) can infect DCs and affect their functions. The 
      aim of this study was to determine the interaction between RSV infection and 
      Dermatophagoides farinae allergen (D. farinae) sensitization on the development 
      of allergy at the DC level. Murine bone marrow-derived DCs were prepared and 
      treated as: control; D. farinae-pulsed DCs (D. farinae-DCs); 
      ultraviolet-inactivated RSV challenged; RSV-infected, D. farinae-pulsed plus 
      ultraviolet-inactivated RSV-challenged; and D. farinae-pulsed plus RSV-infected. 
      In in vitro experiments, we compared the expression of costimulatory molecules 
      and cytokine production between the six groups of DCs. Another group of naive 
      mice were then intranasally inoculated with these DCs, after which intranasal 
      challenge with D. farinae was performed to develop allergic airway inflammation 
      in vivo. In comparison with D. farinae-DCs, D. farinae-pulsed plus RSV-infected 
      DCs showed helper T cell (Th) 1-favored expression of costimulatory molecules and 
      cytokine production. Allergic airway inflammation induced by intranasal 
      instillation of D. farinae-DCs was abrogated when infected with RSV, which was 
      associated with a concomitant suppression of Th2 response in the lung. Our 
      results indicated that RSV suppresses D. farinae-DCs to induce Th2 response both 
      in vitro and in vivo through regulation of expression of surface markers and 
      production of immunoregulatory cytokines.
FAU - Kondo, Yuki
AU  - Kondo Y
AD  - Second Department of Internal Medicine, Nagasaki University School of Medicine, 
      Nagasaki, Japan.
FAU - Matsuse, Hiroto
AU  - Matsuse H
FAU - Machida, Ikuko
AU  - Machida I
FAU - Kawano, Tetsuya
AU  - Kawano T
FAU - Saeki, Sachiko
AU  - Saeki S
FAU - Tomari, Shinya
AU  - Tomari S
FAU - Obase, Yasushi
AU  - Obase Y
FAU - Fukushima, Chizu
AU  - Fukushima C
FAU - Kohno, Shigeru
AU  - Kohno S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20031204
PL  - United States
TA  - Am J Respir Crit Care Med
JT  - American journal of respiratory and critical care medicine
JID - 9421642
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Antigens, Surface)
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Antigens, Dermatophagoides/*immunology
MH  - Antigens, Surface/analysis
MH  - Cells, Cultured
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/*immunology/*virology
MH  - Female
MH  - Lung/pathology
MH  - Lymph Nodes/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Respiratory Hypersensitivity/*immunology/pathology/virology
MH  - Respiratory Syncytial Virus Infections/*immunology/pathology
MH  - Respiratory Syncytial Viruses/*immunology
MH  - Respiratory Tract Infections/immunology/pathology
MH  - Th2 Cells/immunology/metabolism
EDAT- 2003/12/06 05:00
MHDA- 2004/02/27 05:00
CRDT- 2003/12/06 05:00
PHST- 2003/12/06 05:00 [pubmed]
PHST- 2004/02/27 05:00 [medline]
PHST- 2003/12/06 05:00 [entrez]
AID - 200305-663OC [pii]
AID - 10.1164/rccm.200305-663OC [doi]
PST - ppublish
SO  - Am J Respir Crit Care Med. 2004 Feb 15;169(4):494-8. doi: 
      10.1164/rccm.200305-663OC. Epub 2003 Dec 4.