PMID- 14656985
OWN - NLM
STAT- MEDLINE
DCOM- 20040106
LR  - 20120215
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 17
IP  - 15
DP  - 2003 Dec
TI  - Cardiomyocytes overexpressing TNF-alpha attract migration of embryonic stem cells 
      via activation of p38 and c-Jun amino-terminal kinase.
PG  - 2231-9
AB  - Tumor necrosis factor-alpha (TNF-alpha) plays an important role in the 
      pathogenesis of myocardial infarction. Stem cells are able to regenerate 
      infarcted myocardium. This study investigated whether TNF-alpha was able to 
      induce migration of embryonic stem cells (ESCs) in vitro. We used a Transwell 
      assay in which neonatal rat cardiomyocytes, with or without transfection of 
      TNF-alpha cDNA, were plated in the lower compartments and mouse ESCs tagged with 
      green fluorescent protein were added to the upper compartments. TNF-alpha level 
      was significantly increased in the medium of the lower compartments seeded with 
      TNF-alpha-transfected cardiomyocytes. Compared with the controls, overexpression 
      of TNF-alpha significantly enhanced migration of ESCs to the lower compartments. 
      This enhancement was attenuated by preincubation of ESCs with the antibody 
      against the type II TNF-alpha receptor (TNF-RII), but not by the antibody against 
      the type I TNF-alpha receptor (TNF-RI). Western blot analysis showed that the 
      phosphorylated protein levels of p38 and c-Jun amino-terminal kinase (JNK) were 
      significantly increased in TNF-alpha-treated ESCs. Inhibition of the activity of 
      p38 or JNK significantly attenuated TNF-alpha-induced ESC migration. Our data 
      demonstrate that excessive TNF-alpha stimulates TNF-RII and enhances migration of 
      ESCs in vitro. Activation of p38 and JNK is required for TNF-alpha-enhanced ESC 
      migration.
FAU - Chen, Yu
AU  - Chen Y
AD  - Stem Cells Research Laboratory, The Charles A. Dana Research Institute and 
      Harvard-Thorndike Laboratory, Cardiovascular Division, Department of Medicine, 
      Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, 
      Massachusetts 02215, USA.
FAU - Ke, Qingen
AU  - Ke Q
FAU - Yang, Yinke
AU  - Yang Y
FAU - Rana, Jamal S
AU  - Rana JS
FAU - Tang, Jian
AU  - Tang J
FAU - Morgan, James P
AU  - Morgan JP
FAU - Xiao, Yong-Fu
AU  - Xiao YF
LA  - eng
GR  - DA11762/DA/NIDA NIH HHS/United States
GR  - DA12774/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Cell Communication
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Embryo, Mammalian/*cytology
MH  - Enzyme Activation
MH  - JNK Mitogen-Activated Protein Kinases
MH  - MAP Kinase Signaling System
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - Myocytes, Cardiac/*metabolism/physiology
MH  - Rats
MH  - Receptors, Tumor Necrosis Factor/physiology
MH  - Stem Cells/enzymology/*physiology
MH  - Transfection
MH  - Tumor Necrosis Factor-alpha/genetics/metabolism/*physiology
MH  - p38 Mitogen-Activated Protein Kinases
EDAT- 2003/12/06 05:00
MHDA- 2004/01/07 05:00
CRDT- 2003/12/06 05:00
PHST- 2003/12/06 05:00 [pubmed]
PHST- 2004/01/07 05:00 [medline]
PHST- 2003/12/06 05:00 [entrez]
AID - 17/15/2231 [pii]
AID - 10.1096/fj.03-0030com [doi]
PST - ppublish
SO  - FASEB J. 2003 Dec;17(15):2231-9. doi: 10.1096/fj.03-0030com.