PMID- 14657523
OWN - NLM
STAT- MEDLINE
DCOM- 20040831
LR  - 20131121
IS  - 1096-6080 (Print)
IS  - 1096-0929 (Linking)
VI  - 77
IP  - 1
DP  - 2004 Jan
TI  - Serum hormone characterization and exogeneous hormone rescue of 
      bromodichloromethane-induced pregnancy loss in the F344 rat.
PG  - 101-8
AB  - Previously, we demonstrated that bromodichloromethane (BDCM), a drinking water 
      disinfection by-product, causes pregnancy loss in F344 rats when given on 
      gestational days (GD) 6-10, encompassing the luteinizing hormone (LH)-dependent 
      period of pregnancy (GD 7-10). Pregnancy loss, i.e., full-litter resorption, was 
      associated with reduced serum progesterone levels; however, we were unable to 
      identify an effect on serum LH. Here, we reevaluated serum LH levels using the 
      more sensitive technique, DELFIA(R). We further sought to better define the 
      temporal pattern of endocrine disruption caused by BDCM during pregnancy with 
      more frequent sampling. Lastly, we attempted to prevent BDCM-induced pregnancy 
      loss using exogenous progesterone or human chorionic gonadotropin (hCG), an 
      LH-agonist. BDCM, in 10% Alkamuls(R), was dosed at 75 mg/kg/day by gavage to F344 
      rats on GD 6-10 (plug day = GD 0). BDCM-induced pregnancy loss was associated 
      with marked reductions in serum progesterone and LH on GD 10. The decrease in 
      serum LH consistently preceded the decrease in progesterone. In the hormone 
      replacement studies, BDCM and progesterone were administered on GD 6-10, hCG on 
      GD 8-10. BDCM was delivered at 100 mg/kg/day, progesterone at 10 mg/kg twice 
      daily, and hCG at 0.5 IU/0.2 ml/rat. Both progesterone and hCG prevented 
      BDCM-induced pregnancy loss. Thus, BDCM-induced pregnancy loss was associated 
      with marked GD-10 reductions in serum LH and corresponding decreases in 
      progesterone. Furthermore, coadministration of an LH agonist prevented pregnancy 
      loss, supporting the hypothesis that BDCM-induced pregnancy loss in the rat 
      occurs via an LH-mediated mode of action.
FAU - Bielmeier, Susan R
AU  - Bielmeier SR
AD  - Curriculum in Toxicology, University of North Carolina at Chapel Hill, Chapel 
      Hill, North Carolina 25799, USA.
FAU - Best, Deborah S
AU  - Best DS
FAU - Narotsky, Michael G
AU  - Narotsky MG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20031202
PL  - United States
TA  - Toxicol Sci
JT  - Toxicological sciences : an official journal of the Society of Toxicology
JID - 9805461
RN  - 0 (Carcinogens)
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (Trihalomethanes)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 7LN464CH2O (bromodichloromethane)
RN  - 9002-67-9 (Luteinizing Hormone)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Carcinogens/administration & dosage/*toxicity
MH  - Chorionic Gonadotropin/therapeutic use
MH  - Drug Antagonism
MH  - Embryo Loss/*chemically induced/prevention & control
MH  - Female
MH  - Fetal Resorption/*chemically induced/prevention & control
MH  - Luteinizing Hormone/blood
MH  - *Maternal Exposure
MH  - Pregnancy
MH  - Pregnancy, Animal/blood/*drug effects
MH  - Progesterone/therapeutic use
MH  - Rats
MH  - Rats, Inbred F344
MH  - Trihalomethanes/administration & dosage/*toxicity
EDAT- 2003/12/06 05:00
MHDA- 2004/09/01 05:00
CRDT- 2003/12/06 05:00
PHST- 2003/12/06 05:00 [pubmed]
PHST- 2004/09/01 05:00 [medline]
PHST- 2003/12/06 05:00 [entrez]
AID - kfh017 [pii]
AID - 10.1093/toxsci/kfh017 [doi]
PST - ppublish
SO  - Toxicol Sci. 2004 Jan;77(1):101-8. doi: 10.1093/toxsci/kfh017. Epub 2003 Dec 2.