PMID- 14658021
OWN - NLM
STAT- MEDLINE
DCOM- 20040715
LR  - 20160512
IS  - 1618-2642 (Print)
IS  - 1618-2642 (Linking)
VI  - 378
IP  - 3
DP  - 2004 Feb
TI  - Integrated procedure for determination of endocrine-disrupting activity in 
      surface waters and sediments by use of the biological technique recombinant yeast 
      assay and chemical analysis by LC-ESI-MS.
PG  - 697-708
AB  - An integrated procedure using mass spectrometry and molecular biology for 
      determination of estrogenicity in natural waters and sediments is reported. 
      Solid-phase extraction (SPE) and pressurized-liquid extraction (PLE), 
      respectively, were used for isolation of endocrine-disrupting compounds (EDC) 
      from surface waters and sediments, followed by liquid chromatography-mass 
      spectrometry using an electrospray interface (LC-ESI-MS). Twenty seven EDC were 
      determined: non-ionic surfactants (nonylphenol ethoxylate), alkylphenols (e.g. 
      nonylphenol and octylphenol), bisphenol A, phthalates, and natural and synthetic 
      steroid sex hormones. Limits of detection varied from 0.02 to 0.22 microg L(-1) 
      and from 1 to 10 microg kg(-1) in water and sediments, respectively. Recoveries 
      ranged from 65 to 125% and 73 to 97% for waters and sediments, respectively. In 
      addition to LC-ESI-MS determination, extracts obtained by SPE and PLE were 
      analyzed by the recombinant yeast assay (RYA) to assess total estrogenic 
      activity. This bioassay detects natural estrogens and xenoestrogens, producing a 
      quantitative measurement of EDC irrespective of the identity of the chemical 
      responsible for the activity. As a novelty, a relative estrogenicity factor was 
      determined for 19 analytes with EC(50) values ranging from 10(-10) to 10(-9) mol 
      L(-1) for synthetic estrogens, from 10(-7) to 10(-5) mol L(-1) for alkylphenol 
      derivatives, and from 10(-5) to 10(-4) mol L(-1) for phthalates and 
      benzothiazoles. By use of this integrated chemical-ecotoxicological approach good 
      correlation was usually established between chemical composition and estrogenic 
      effects for surface water and sediment samples from Portugal. Estrogenic activity 
      observed was mainly attributed to the presence of nonylphenolic compounds (with 
      concentrations of NP ranging from 0.1 up to 44 microg L(-1) in waters and up to 
      1172 microg kg(-1) in sediments), and to the sporadic presence of estrogens, 
      detected at ng L(-1) levels.
FAU - Cespedes, Raquel
AU  - Cespedes R
AD  - Department of Environmental Chemistry, IIQAB-CSIC, Jordi Girona 18-26, 08034 
      Barcelona, Spain.
FAU - Petrovic, Mira
AU  - Petrovic M
FAU - Raldua, Demetrio
AU  - Raldua D
FAU - Saura, Ursula
AU  - Saura U
FAU - Pina, Benjamin
AU  - Pina B
FAU - Lacorte, Silvia
AU  - Lacorte S
FAU - Viana, Paula
AU  - Viana P
FAU - Barcelo, Damia
AU  - Barcelo D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20031205
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Water Pollutants, Chemical)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Endocrine Glands/*drug effects
MH  - Geologic Sediments/chemistry
MH  - Recombination, Genetic
MH  - Reference Standards
MH  - Saccharomyces cerevisiae/*genetics
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Water Pollutants, Chemical/*analysis/toxicity
EDAT- 2003/12/06 05:00
MHDA- 2004/07/16 05:00
CRDT- 2003/12/06 05:00
PHST- 2003/05/23 00:00 [received]
PHST- 2003/09/12 00:00 [revised]
PHST- 2003/09/22 00:00 [accepted]
PHST- 2003/12/06 05:00 [pubmed]
PHST- 2004/07/16 05:00 [medline]
PHST- 2003/12/06 05:00 [entrez]
AID - 10.1007/s00216-003-2303-5 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2004 Feb;378(3):697-708. doi: 10.1007/s00216-003-2303-5. Epub 
      2003 Dec 5.