PMID- 14658760
OWN - NLM
STAT- MEDLINE
DCOM- 20040812
LR  - 20161124
IS  - 1521-6543 (Print)
IS  - 1521-6543 (Linking)
VI  - 55
IP  - 9
DP  - 2003 Sep
TI  - Identification and characterization of a serum protein homologous to alpha-type 
      phospholipase A2 inhibitor (PLIalpha) from a nonvenomous snake, Elaphe 
      quadrivirgata.
PG  - 539-45
AB  - From a liver cDNA library prepared from a nonvenomous striated snake, Elaphe 
      quadrivirgata, we isolated a cDNA encoding a novel protein, PLIalpha-like protein 
      (PLIalpha-LP), having approximately 70% sequence identities with the alpha-type 
      phospholipase A2 (PLA2) inhibitors (PLIalpha(s)) previously purified from the 
      venomous snakes Agkistrodon blomhoffii siniticus and Trimeresurus flavoviridis. 
      Since the PLI-LP with a highly conserved C-type lectin-like domain (CTLD) would 
      be predicted to function as a PLA2 inhibitor, we purified this protein from E. 
      quadrivirgata serum by sequential chromatography on Hi-trap Blue, Mono Q, and 
      Superdex 200 columns. The purified 51-kDa protein with PLIalpha-like 
      immunoreactivity was found to be a trimer of 18-kDa PLIalpha-LP, which was 
      comparable to the trimeric structure of PLIalpha. But, unexpectedly, this protein 
      did not show any inhibitory activity against various snake venom PLA2s. 
      Furthermore, it did not inhibit the endogenous PLA2 activities in various tissue 
      homogenates prepared from this snake. Lack of the inhibitory activity in 
      PLIalpha-LP may provide important information concerning the structure-function 
      relationships of PLIalpha.
FAU - Okumura, Kohji
AU  - Okumura K
AD  - Department of Biochemistry, Osaka University of Pharmaceutical Sciences, 4-20-1 
      Nasahara, Takatsuki, Osaka 569-1094, Japan.
FAU - Inoue, Seiji
AU  - Inoue S
FAU - Ikeda, Kiyoshi
AU  - Ikeda K
FAU - Hayashi, Kyozo
AU  - Hayashi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - IUBMB Life
JT  - IUBMB life
JID - 100888706
RN  - 0 (Blood Proteins)
RN  - 0 (PLIalpha)
RN  - EC 3.1.1.32 (Phospholipases A)
RN  - EC 3.1.1.4 (Phospholipases A2)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blood Proteins/*genetics/*isolation & purification/metabolism
MH  - Liver/metabolism
MH  - Molecular Sequence Data
MH  - Phospholipases A/antagonists & inhibitors
MH  - Phospholipases A2
MH  - Sequence Homology
MH  - Snakes/genetics/*metabolism
EDAT- 2003/12/09 05:00
MHDA- 2004/08/13 05:00
CRDT- 2003/12/09 05:00
PHST- 2003/12/09 05:00 [pubmed]
PHST- 2004/08/13 05:00 [medline]
PHST- 2003/12/09 05:00 [entrez]
AID - 10.1080/15216540310001620995 [doi]
PST - ppublish
SO  - IUBMB Life. 2003 Sep;55(9):539-45. doi: 10.1080/15216540310001620995.