PMID- 14658844
OWN - NLM
STAT- MEDLINE
DCOM- 20040209
LR  - 20191210
IS  - 0001-723X (Print)
IS  - 0001-723X (Linking)
VI  - 47
IP  - 3
DP  - 2003
TI  - Detection of immediate early protein ICP27/IE63 and thymidine kinase in the 
      course of reactivation of latent herpes simplex virus 1 infection.
PG  - 159-65
AB  - We followed the kinetics of reactivation of latent Herpes simplex virus 1 (HSV-1) 
      infection established in rabbits by corneal route. The corresponding trigeminal 
      ganglia (TG) were cultured and the culture medium was examined at daily intervals 
      for release of infectious virus. Sections from the cultured TG fragments were 
      stained with antisera against non-structural proteins such as the immediate early 
      (IE) protein ICP27 and the early (E) proteins thymidine kinase (TK), the large 
      subunit of ribonucleotide reductase (RR1), the ori-binding protein OBP and with a 
      human serum obtained from volunteers immunized with an experimental subunit HSV-1 
      envelope (env) vaccine containing late structural proteins gB1, gC1, gD1 and gG1 
      (env antiserum). By indirect immunofluorescence (IF) test, ICP27 was detected in 
      a few neurons from day 1 post explantation (p.e.), while TK was observed in 
      neurons from day 2 p.e. Fluorescence with the human env antiserum was seen at day 
      3 p.e. The RR1 and OBP antisera stained productively infected Vero cells from 3 
      and 4 hrs post inoculation (p.i.), respectively. However, these sera showed no IF 
      in cultured ganglion fragments at any interval examined. Our results showed the 
      same cascade of HSV-1 IE and E protein expression during productive infection and 
      reactivation in vitro.
FAU - Durmanova, V
AU  - Durmanova V
AD  - Institute of Virology, Slovak Academy of Sciences, Dubravska cesta 9, 845 05 
      Bratislava 4, Slovak Republic. viruraj@savba.sk
FAU - Rajcani, J
AU  - Rajcani J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Slovakia
TA  - Acta Virol
JT  - Acta virologica
JID - 0370401
RN  - 0 (ICP27 protein, human herpesvirus 1)
RN  - 0 (Immediate-Early Proteins)
RN  - EC 2.7.1.21 (Thymidine Kinase)
SB  - IM
MH  - Animals
MH  - Chlorocebus aethiops
MH  - Cornea/virology
MH  - Fluorescent Antibody Technique
MH  - Herpesvirus 1, Human/enzymology/pathogenicity/*physiology
MH  - Humans
MH  - Immediate-Early Proteins/*metabolism
MH  - Keratitis, Herpetic/virology
MH  - Rabbits
MH  - Thymidine Kinase/*metabolism
MH  - Trigeminal Ganglion/virology
MH  - Vero Cells
MH  - *Virus Activation
MH  - Virus Latency
EDAT- 2003/12/09 05:00
MHDA- 2004/02/11 05:00
CRDT- 2003/12/09 05:00
PHST- 2003/12/09 05:00 [pubmed]
PHST- 2004/02/11 05:00 [medline]
PHST- 2003/12/09 05:00 [entrez]
PST - ppublish
SO  - Acta Virol. 2003;47(3):159-65.