PMID- 14659561
OWN - NLM
STAT- MEDLINE
DCOM- 20040329
LR  - 20190911
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 98
IP  - 2
DP  - 2003 Dec
TI  - A 25 kDa cleavage product of polypyrimidine tract binding protein (PTB) present 
      in mouse tissues prevents PTB binding to the 5' untranslated region and inhibits 
      translation of hepatitis A virus RNA.
PG  - 141-9
AB  - The 5' untranslated region (5'UTR) of the hepatitis A virus (HAV) genomic RNA 
      contains an internal ribosome entry site (IRES) which interacts with various 
      cellular proteins and facilitates cap-independent translation. We report the 
      interaction of a 25kDa protein (p25), present in certain murine tissues and most 
      abundantly in mouse kidney, with the HAV 5'UTR. This protein was found to be a 
      cleavage product of the polypyrimidine tract-binding protein (PTB) and competed 
      with it for binding to the HAV 5'UTR RNA. The binding site of p25 overlapped with 
      the reported binding site of PTB. Exogenous addition of partially purified p25 to 
      in vitro translation reactions resulted in the inhibition of HAV IRES-mediated 
      translation, which could be rescued by the addition of purified PTB. These 
      results suggest that p25 is a cleavage product of PTB which binds to the HAV IRES 
      and antagonizes the translation-stimulating activity of PTB. The presence of the 
      25kDa cleavage product of PTB may therefore play a role in the inhibition of HAV 
      IRES-mediated translation in mouse tissues.
FAU - Venkatramana, Musturi
AU  - Venkatramana M
AD  - Department of Microbiology and Cell Biology, Indian Institute of Science, 560012 
      Bangalore, India.
FAU - Ray, Partho Sarothi
AU  - Ray PS
FAU - Chadda, Ankita
AU  - Chadda A
FAU - Das, Saumitra
AU  - Das S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Peptide Fragments)
RN  - 0 (RNA-Binding Proteins)
RN  - 139076-35-0 (Polypyrimidine Tract-Binding Protein)
SB  - IM
MH  - 5' Untranslated Regions/*drug effects
MH  - Animals
MH  - HeLa Cells
MH  - Hepatitis A virus/*drug effects/genetics
MH  - Humans
MH  - Mice
MH  - Molecular Weight
MH  - Peptide Fragments/pharmacology
MH  - Polypyrimidine Tract-Binding Protein/*pharmacology
MH  - Protein Biosynthesis/*drug effects
MH  - RNA-Binding Proteins/metabolism
MH  - Regulatory Sequences, Nucleic Acid
EDAT- 2003/12/09 05:00
MHDA- 2004/03/30 05:00
CRDT- 2003/12/09 05:00
PHST- 2003/12/09 05:00 [pubmed]
PHST- 2004/03/30 05:00 [medline]
PHST- 2003/12/09 05:00 [entrez]
AID - S0168170203002788 [pii]
AID - 10.1016/j.virusres.2003.09.004 [doi]
PST - ppublish
SO  - Virus Res. 2003 Dec;98(2):141-9. doi: 10.1016/j.virusres.2003.09.004.