PMID- 14659833
OWN - NLM
STAT- MEDLINE
DCOM- 20040301
LR  - 20190901
IS  - 0165-0270 (Print)
IS  - 0165-0270 (Linking)
VI  - 131
IP  - 1-2
DP  - 2003 Dec 30
TI  - Primary culture of cortical neurons, type-1 astrocytes, and microglial cells from 
      cynomolgus monkey (Macaca fascicularis) fetuses.
PG  - 133-40
AB  - We established selective primary cultures of neurons, astrocytes, and microglial 
      cells from cryopreserved fetal cerebral cortex of cynomolgus monkeys (Macaca 
      fascicularis). At 14 days in serum-containing medium, the cell cultures of the 
      fetal cerebral cortex consisted primarily of neurons, astrocytes, and floating 
      microglial cells. At 21 days, we observed a small number of myelin basic protein 
      (MBP)-positive oligodendrocytes. The addition of cytosine arabinoside (a 
      selective DNA synthesis inhibitor) at 2 days in culture eliminated proliferative 
      glial cells, allowing adequate numbers of neurons to survive selectively. A 
      chemically defined serum-free medium successfully supported neuronal survival at 
      a level equivalent to that supported by the serum-containing medium. 
      Brain-derived neurotrophic factor (BDNF) significantly affected the survival of 
      primate neurons. Glutamate induced a significant degree of neuronal cell death 
      against primate neurons and MK-801, a selective N-methyl-D-aspartate receptor 
      (NMDAR) antagonist, blocked cell death, which suggests that primate cortical 
      neurons have NMDAR and the glutamate-induced cell toxicity is mediated by NMDAR. 
      In the serum-free medium, type-1 astrocytes responded to dibutyryl cyclic AMP and 
      showed a process-bearing morphology. The growth of type-1 astrocytes in the 
      serum-free medium was stimulated by epidermal growth factor (EGF), basic 
      fibroblast growth factor (bFGF), and hydrocortisone, which are known growth 
      factors in rat type-1 astrocytes. Cultured microglial cells expressed CD68, a 
      monocyte marker. Macrophage-colony stimulating factor (M-CSF) stimulated 
      microglial cell growth in the serum-free medium. These selective primary culture 
      systems of primate cerebral cortical cells will be useful in issues involving 
      species specificity in neuroscience.
FAU - Negishi, Takayuki
AU  - Negishi T
AD  - Department of Biomedical Science, Graduate School of Agricultural and Life 
      Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. 
      taka-u@yayoi.club.ne.jp
FAU - Ishii, Yoshiyuki
AU  - Ishii Y
FAU - Kyuwa, Shigeru
AU  - Kyuwa S
FAU - Kuroda, Yoichiro
AU  - Kuroda Y
FAU - Yoshikawa, Yasuhiro
AU  - Yoshikawa Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - J Neurosci Methods
JT  - Journal of neuroscience methods
JID - 7905558
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD68 antigen, human)
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Myelin Basic Protein)
RN  - 04079A1RDZ (Cytarabine)
RN  - 3616-08-8 (Cyclic CMP)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 62031-54-3 (Fibroblast Growth Factors)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 64649-87-2 (dibutyryl cyclic-3',5'-cytidine monophosphate)
RN  - 6LR8C1B66Q (Dizocilpine Maleate)
SB  - IM
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Antigens, Differentiation, Myelomonocytic/metabolism
MH  - Astrocytes/drug effects/*physiology
MH  - Cell Culture Techniques/*methods
MH  - Cell Death/physiology
MH  - Cell Division/drug effects/physiology
MH  - Cells, Cultured
MH  - Cerebral Cortex/*cytology/drug effects/metabolism
MH  - Culture Media, Serum-Free/pharmacology
MH  - Cyclic CMP/*analogs & derivatives/pharmacology
MH  - Cytarabine/pharmacology
MH  - Dizocilpine Maleate/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Epidermal Growth Factor/pharmacology
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - Fetus/*cytology
MH  - Fibroblast Growth Factors/pharmacology
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Glutamic Acid/pharmacology
MH  - Immunohistochemistry
MH  - Immunosuppressive Agents/pharmacology
MH  - Macaca fascicularis
MH  - Microglia/drug effects/*physiology
MH  - Microtubule-Associated Proteins/metabolism
MH  - Myelin Basic Protein/metabolism
MH  - Neurons/drug effects/*physiology
EDAT- 2003/12/09 05:00
MHDA- 2004/03/03 05:00
CRDT- 2003/12/09 05:00
PHST- 2003/12/09 05:00 [pubmed]
PHST- 2004/03/03 05:00 [medline]
PHST- 2003/12/09 05:00 [entrez]
AID - S0165027003002681 [pii]
AID - 10.1016/j.jneumeth.2003.08.006 [doi]
PST - ppublish
SO  - J Neurosci Methods. 2003 Dec 30;131(1-2):133-40. doi: 
      10.1016/j.jneumeth.2003.08.006.