PMID- 14659882
OWN - NLM
STAT- MEDLINE
DCOM- 20040217
LR  - 20211203
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 323
DP  - 2003 Dec 24
TI  - Specific alternative HOX11 transcripts are expressed in paediatric neural tumours 
      and T-cell acute lymphoblastic leukaemia.
PG  - 89-99
AB  - HOX11 is a proto-oncogene, which is silent in normal mature T-cells, while being 
      aberrantly activated in T-cell acute lymphoblastic leukaemia (T-ALL) by 
      translocations t(10;14)(q24;q11) or t(7;10)(q35;q24). Although many oncogenes are 
      expressed in alternative forms in cancer, thus far, only one form of the human 
      HOX11 transcript has been reported. We describe here the identification of three 
      alternative transcripts of the HOX11 proto-oncogene, expressed in primary T-ALL 
      specimens. Using rapid amplification of cDNA ends (RACE) and targeted RT-PCR, we 
      have sequenced 23 individual cDNA clones characterising these novel transcripts. 
      Northern hybridisation identified particular novel exons expressed in T-ALL, 
      which are not expressed in normal T-cells. To date, aberrant expression of HOX11 
      has only been associated with leukaemia. Our survey of a range of neuroblastoma 
      and primitive neuroectodermal tumour (PNET) cell lines demonstrated the 
      expression of these novel HOX11 transcripts in tumours of neural origin, while 
      their expression was not detected in normal brain tissues. Strikingly, the 
      dominant transcript in these neural tumour cell lines is more than 1 kb larger 
      than the dominant transcript in T-ALL. These observations, combined with sequence 
      data from several EST clones derived from medulloblastoma cDNA libraries, support 
      a new hypothesis that HOX11 may also function as a neural oncogene or brain 
      tumour marker.
FAU - Watt, Paul M
AU  - Watt PM
AD  - Division of Children's Leukaemia and Cancer Research, Telethon Institute for 
      Child Health Research and Centre for Child Health Research, The University of 
      Western Australia, P O Box 855,Western Australia 6872, West Perth, Australia.
FAU - Hoffmann, Katrin
AU  - Hoffmann K
FAU - Greene, Wayne K
AU  - Greene WK
FAU - Brake, Rachael L
AU  - Brake RL
FAU - Ford, Jette
AU  - Ford J
FAU - Kees, Ursula R
AU  - Kees UR
LA  - eng
SI  - GENBANK/CB185049
SI  - GENBANK/CB185050
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA, Complementary)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (MAS1 protein, human)
RN  - 0 (Oncogene Proteins)
RN  - 0 (Proto-Oncogene Mas)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 143275-75-6 (TLX1 protein, human)
SB  - IM
MH  - *Alternative Splicing
MH  - Blotting, Northern
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Child
MH  - DNA, Complementary/chemistry/genetics
MH  - Expressed Sequence Tags
MH  - *Gene Expression Regulation, Neoplastic
MH  - Homeodomain Proteins/*genetics
MH  - Humans
MH  - Leukemia-Lymphoma, Adult T-Cell/*genetics/pathology
MH  - Molecular Sequence Data
MH  - Neuroblastoma/*genetics/pathology
MH  - Oncogene Proteins/*genetics
MH  - Proto-Oncogene Mas
MH  - Proto-Oncogene Proteins
MH  - Sequence Analysis, DNA
MH  - Transcription, Genetic
EDAT- 2003/12/09 05:00
MHDA- 2004/02/18 05:00
CRDT- 2003/12/09 05:00
PHST- 2003/12/09 05:00 [pubmed]
PHST- 2004/02/18 05:00 [medline]
PHST- 2003/12/09 05:00 [entrez]
AID - S0378111903008990 [pii]
AID - 10.1016/j.gene.2003.09.001 [doi]
PST - ppublish
SO  - Gene. 2003 Dec 24;323:89-99. doi: 10.1016/j.gene.2003.09.001.