PMID- 14660057
OWN - NLM
STAT- MEDLINE
DCOM- 20040428
LR  - 20171116
IS  - 1025-3890 (Print)
IS  - 1025-3890 (Linking)
VI  - 6
IP  - 4
DP  - 2003 Dec
TI  - Stress applied during primary immunization affects the secondary humoral immune 
      response in the rat: involvement of opioid peptides.
PG  - 247-58
AB  - The effect of unpredictable, inescapable and uncontrollable electric tail shocks 
      (ES) on the humoral immune response to bovine serum albumin (BSA) was 
      investigated in the rat. Contributions of the procedures that accompany shock 
      delivery, such as witnessing the ES procedure (stress witnessing, SW) and 
      exposure to the apparatus for shock delivery (apparatus control, AC) to the 
      changes in specific immunity induced by ES were also tested. All procedures were 
      applied during primary and/or secondary immunization. It was demonstrated that 
      exposure to ES during primary immunization with BSA significantly suppressed 
      specific anti-BSA antibody production after secondary and tertiary immunization 
      with the same antigen. Exposure to the SW procedure during primary immunization 
      with BSA enhanced the specific antibody level after secondary immunization, while 
      exposure to the apparatus alone did not influence the development of either the 
      primary or secondary humoral immune response to BSA. Both ES-induced suppression 
      and SW-induced potentiation of the humoral immune response were partially 
      inhibited by prior treatment with the opioid receptor antagonist naloxone. 
      Additionally, treatments with the opioid peptides methionine- and 
      leucine-enkephalin decreased anti-BSA antibody level, mimicking to some extent 
      the effects of ES. It is suggested that ES and endogenous opioid peptides had 
      long-term effects on humoral immunity through mechanisms involving immunologic 
      memory.
FAU - Stanojevic, Stanislava
AU  - Stanojevic S
AD  - Immunology Research Center "Branislav Jankovic, Institute of Immunology and 
      Virology, Belgrade, Serbia. canac@eunet.yu
FAU - Dimitrijevic, Mirjana
AU  - Dimitrijevic M
FAU - Kovacevic-Jovanovic, Vesna
AU  - Kovacevic-Jovanovic V
FAU - Miletic, Tatjana
AU  - Miletic T
FAU - Vujic, Vesna
AU  - Vujic V
FAU - Radulovic, Jelena
AU  - Radulovic J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Stress
JT  - Stress (Amsterdam, Netherlands)
JID - 9617529
RN  - 0 (Antibodies)
RN  - 0 (Narcotic Antagonists)
RN  - 0 (Opioid Peptides)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 36B82AMQ7N (Naloxone)
RN  - 58569-55-4 (Enkephalin, Methionine)
RN  - 58822-25-6 (Enkephalin, Leucine)
SB  - IM
MH  - Animals
MH  - Antibodies/analysis
MH  - Antibody Formation
MH  - Cattle
MH  - Electroshock
MH  - Enkephalin, Leucine/pharmacology
MH  - Enkephalin, Methionine/pharmacology
MH  - *Immunity
MH  - *Immunization
MH  - Immunization, Secondary
MH  - Male
MH  - Naloxone/pharmacology
MH  - Narcotic Antagonists/pharmacology
MH  - Opioid Peptides/antagonists & inhibitors/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Serum Albumin, Bovine/immunology
MH  - Stress, Physiological/etiology/*immunology
MH  - Stress, Psychological/immunology
EDAT- 2003/12/09 05:00
MHDA- 2004/04/29 05:00
CRDT- 2003/12/09 05:00
PHST- 2003/12/09 05:00 [pubmed]
PHST- 2004/04/29 05:00 [medline]
PHST- 2003/12/09 05:00 [entrez]
AID - T930BCJJNM08M2HK [pii]
AID - 10.1080/1025389032000114515 [doi]
PST - ppublish
SO  - Stress. 2003 Dec;6(4):247-58. doi: 10.1080/1025389032000114515.