PMID- 14662319
OWN - NLM
STAT- MEDLINE
DCOM- 20040209
LR  - 20191026
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 31
IP  - 12
DP  - 2003 Dec
TI  - A novel strategy for allogeneic stem cell transplantation: perfusion method plus 
      intra-bone marrow injection of stem cells.
PG  - 1142-6
AB  - Using long bones of cynomolgus monkeys, we have recently developed a new 
      "Perfusion Method (PM)" for harvesting bone marrow cells (BMCs) while minimizing 
      the contamination of BMCs with T cells from the peripheral blood. When thus 
      collected BMCs, which contain not only pluripotent hemopoietic stem cells 
      (P-HSCs) but also mesenchymal stem cells (MSCs), are directly injected into the 
      bone marrow cavity of recipients (intra-bone marrow BMT: "IBM-BMT"), the 
      donor-derived hemopoietic cells quickly recover even when the radiation doses 
      used as the conditioning regimen are reduced. Recipient mice, rats, and even 
      monkeys show neither graft-vs-host disease (GVHD) nor graft failure. In this 
      article, we discuss why this new method (PM+IBM-BMT) may become a valuable 
      strategy for allogeneic stem cell (both P-HSC and MSC) transplantation.
FAU - Ikehara, Susumu
AU  - Ikehara S
AD  - Kansai Medical University, 10-15 Fumizono-cho, Moriguchi City, Osaka 570-8506, 
      Japan. ikehara@takii.kmu.ac.jp
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
SB  - IM
MH  - Animals
MH  - Bone Marrow
MH  - Bone Marrow Transplantation/methods
MH  - Hematopoietic Stem Cell Transplantation/*methods
MH  - Injections/methods
MH  - Macaca fascicularis
MH  - Mice
MH  - Perfusion/methods
MH  - Transplantation, Homologous
RF  - 29
EDAT- 2003/12/10 05:00
MHDA- 2004/02/11 05:00
CRDT- 2003/12/10 05:00
PHST- 2003/12/10 05:00 [pubmed]
PHST- 2004/02/11 05:00 [medline]
PHST- 2003/12/10 05:00 [entrez]
AID - S0301472X03003023 [pii]
AID - 10.1016/j.exphem.2003.08.020 [doi]
PST - ppublish
SO  - Exp Hematol. 2003 Dec;31(12):1142-6. doi: 10.1016/j.exphem.2003.08.020.