PMID- 14662798
OWN - NLM
STAT- MEDLINE
DCOM- 20040825
LR  - 20220310
IS  - 0196-3635 (Print)
IS  - 0196-3635 (Linking)
VI  - 25
IP  - 1
DP  - 2004 Jan-Feb
TI  - Chromosomal abnormalities and y chromosome microdeletions in infertile men with 
      varicocele and idiopathic infertility of South Indian origin.
PG  - 147-53
AB  - Various factors cause spermatogenesis arrest in men and, in a large number of 
      cases, the underlying reason still remains unknown. Little attention is paid to 
      determining the genetic defects of varicocele-related infertility. The objective 
      of our present study was to investigate the chromosomal abnormalities and Y 
      chromosome microdeletions in infertile men of South Indian origin with varicocele 
      and idiopathic infertility. Metaphase chromosomes of 251 infertile men with 
      varicocele and unexplained infertility were analyzed using Giemsa-Trypsin-Giemsa 
      (GTG) banding and fluorescence in situ hybridization (FISH). The microdeletions 
      in 6 genes and 18 sequence-tagged-sites (STS) in the Yq region were screened 
      using polymerase chain reaction (PCR) techniques. Out of 251 infertile men, 57 
      (22.7%) men were with varicocele, of which 8.77% were azoospermic, 26.31% were 
      severely oligozoospermic, 21.05% were mildly oligozoospermic, and 43.85% were 
      oligoasthenoteratozoospermic (OAT), and 194 (77.29%), with idiopathic 
      infertility, of which 51% were azoospermic, 13.40% were severely oligozoospermic, 
      19.07% were mildly oligozoospermic, and 16.4% were with OAT. Genetic defects were 
      observed in 38 (15.13%) infertile individuals, including 14 (24.56%) men with 
      varicocele and 24 (12.37%) men with idiopathic infertility. The frequencies of 
      chromosomal defects in varicocele and idiopathic infertility were 19.3% and 
      8.76%, respectively, whereas Y chromosome microdeletions were 5.26% and 3.60%, 
      respectively. Overall rate of incidence of chromosomal anomalies and 
      microdeletions in 251 infertile men were 11.5% and 3.98%, respectively, 
      indicating a very significant higher association of genetic defects with 
      varicocele than idiopathic male infertility. Our data also demonstrate that, 
      among infertile men with varicocele, severely oligozoospermic and OAT men with 
      varicocele have higher incidences of genetic defects than mildly oligozoospermic 
      and azoospermic men.
FAU - Rao, Lakshmi
AU  - Rao L
AD  - Centre for Cellular and Molecular Biology, Hyderabad, India.
FAU - Babu, Arvind
AU  - Babu A
FAU - Kanakavalli, Murthy
AU  - Kanakavalli M
FAU - Padmalatha, Venkata
AU  - Padmalatha V
FAU - Singh, Amarpal
AU  - Singh A
FAU - Singh, Prashant Kumar
AU  - Singh PK
FAU - Deenadayal, Mamata
AU  - Deenadayal M
FAU - Singh, Lalji
AU  - Singh L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Androl
JT  - Journal of andrology
JID - 8106453
SB  - IM
MH  - Adult
MH  - *Chromosome Aberrations
MH  - *Chromosome Deletion
MH  - *Chromosomes, Human, Y
MH  - Genetic Testing
MH  - Humans
MH  - India
MH  - Infertility, Male/*genetics
MH  - Male
MH  - Middle Aged
MH  - Varicocele/*genetics
EDAT- 2003/12/10 05:00
MHDA- 2004/08/26 05:00
CRDT- 2003/12/10 05:00
PHST- 2003/12/10 05:00 [pubmed]
PHST- 2004/08/26 05:00 [medline]
PHST- 2003/12/10 05:00 [entrez]
AID - 10.1002/j.1939-4640.2004.tb02770.x [doi]
PST - ppublish
SO  - J Androl. 2004 Jan-Feb;25(1):147-53. doi: 10.1002/j.1939-4640.2004.tb02770.x.