PMID- 14663510
OWN - NLM
STAT- MEDLINE
DCOM- 20040923
LR  - 20220317
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 46
IP  - 6
DP  - 2003 Dec
TI  - Visualizing DNA domains and sequences by microscopy: a fifty-year history of 
      molecular cytogenetics.
PG  - 943-6
AB  - This short review presents a historical perspective of chromosome research during 
      the last 50 years. It shows how molecular knowledge and technology of DNA entered 
      cytogenetics step by step making it now daily practice in almost every modern 
      chromosome lab. A crucial milestone in these decades has been the development of 
      in situ protocols by Pardue and Gall, among others, initially only with isotopic 
      labels, and without fluorescence microscopy and sophisticated detection systems. 
      But these very first in situ hybridizations played a decisive role in the 
      discovery of chromosome banding profiles, which were obtained under specific 
      chemical, physical, or enzymatic conditions, thus effecting stainability of 
      specific chromosome regions. In the decades thereafter, numerous technical 
      improvements were achieved leading to complex multi-colour fluorescence in situ 
      hybridization (FISH) protocols for mammals, plants, and insects. Highly improved 
      detection systems of the FISH signals further allowed detection of DNA targets of 
      up to 50 bp, whereas other protocols, which were developed to stretch chromatin 
      fibres to the full length of native DNA, improved spatial resolution of adjacent 
      targets in the light microscope to 1 kb.
FAU - de Jong, Hans
AU  - de Jong H
AD  - Wageningen University, Laboratory of Genetics, The Netherlands. 
      hans.dejong@wur.nl
LA  - eng
PT  - Journal Article
PT  - Review
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (Fluorescent Dyes)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Cytogenetics/methods/trends
MH  - DNA/chemistry/*genetics
MH  - Fluorescent Dyes/chemistry
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microscopy, Fluorescence/*methods
RF  - 33
EDAT- 2003/12/10 05:00
MHDA- 2004/09/24 05:00
CRDT- 2003/12/10 05:00
PHST- 2003/12/10 05:00 [pubmed]
PHST- 2004/09/24 05:00 [medline]
PHST- 2003/12/10 05:00 [entrez]
AID - g03-107 [pii]
AID - 10.1139/g03-107 [doi]
PST - ppublish
SO  - Genome. 2003 Dec;46(6):943-6. doi: 10.1139/g03-107.