PMID- 14663530 OWN - NLM STAT- MEDLINE DCOM- 20040923 LR - 20161020 IS - 0831-2796 (Print) IS - 0831-2796 (Linking) VI - 46 IP - 6 DP - 2003 Dec TI - Distribution of highly repeated DNA sequences in species of the genus Lens Miller. PG - 1118-24 AB - Multiple-target fluorescence in situ hybridization (FISH) was applied on mitotic chromosomes of seven Lens taxa using two highly repetitive sequences (pLc30 and pLc7) isolated from the cultivated lentil and the multigene families for the 18S-5.8S-25S (pTa71) and 5S rRNA (pTa794) from wheat simultaneously as probes. The number and location of pLc30 and pLc7 sites on chromosomes varied markedly among the species, whereas the hybridization pattern of 5S rDNA and 18S-5.8S-25S rDNA was less variable. In general, each species showed a typical FISH karyotype and few differences were observed among accessions belonging to the same species, except for the accessions of Lens odemensis. The most similar FISH karyotype to the cultivated lentil is that of Lens culinaris subsp. orientalis, whereas Lens nigricans and Lens tomentosus are the two species that showed the most divergent FISH patterns compared with all taxa for number and location of pLc30 and 18S-5.8S-25S rDNA sites. FAU - Galasso, Incoronata AU - Galasso I AD - Istituto di Genetica Vegetale, CNR, Bari, Italy. tella.galasso@igv.cnr.it LA - eng PT - Journal Article PL - Canada TA - Genome JT - Genome JID - 8704544 RN - 0 (DNA, Plant) RN - 0 (DNA, Ribosomal) RN - 0 (RNA, Ribosomal) RN - 0 (RNA, Ribosomal, 18S) RN - 0 (RNA, Ribosomal, 5.8S) RN - 0 (RNA, Ribosomal, 5S) RN - 130527-23-0 (RNA, ribosomal, 25S) SB - IM MH - Chromosomes, Plant/genetics MH - DNA, Plant/genetics MH - DNA, Ribosomal/*genetics MH - Fabaceae/*genetics MH - In Situ Hybridization, Fluorescence MH - Karyotyping MH - RNA, Ribosomal/genetics MH - RNA, Ribosomal, 18S/genetics MH - RNA, Ribosomal, 5.8S/genetics MH - RNA, Ribosomal, 5S/genetics MH - Repetitive Sequences, Nucleic Acid/*genetics MH - Species Specificity EDAT- 2003/12/10 05:00 MHDA- 2004/09/24 05:00 CRDT- 2003/12/10 05:00 PHST- 2003/12/10 05:00 [pubmed] PHST- 2004/09/24 05:00 [medline] PHST- 2003/12/10 05:00 [entrez] AID - g03-077 [pii] AID - 10.1139/g03-077 [doi] PST - ppublish SO - Genome. 2003 Dec;46(6):1118-24. doi: 10.1139/g03-077.