PMID- 1466394
OWN - NLM
STAT- MEDLINE
DCOM- 19930115
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 141
IP  - 6
DP  - 1992 Dec
TI  - Trisomy 12 in pediatric granulosa-stromal cell tumors. Demonstration by a 
      modified method of fluorescence in situ hybridization on paraffin-embedded 
      material.
PG  - 1265-9
AB  - The use of fluorescence in situ hybridization (FISH) to detect chromosomal 
      abnormalities has many applications. Use of FISH on archival, paraffin-embedded 
      material has been limited to microscopic sections. We have carried out FISH on 
      preparations of disaggregated nuclei obtained from paraffin-embedded tissue to 
      evaluate chromosome 12 copy number in granulosa-stromal cell neoplasms occurring 
      in infants, children, and adolescents. Trisomy 12 was detected in the majority of 
      cells from three of four juvenile granulosa cell tumors (three ovarian and one 
      testicular) and one malignant granulosa cell tumor. Tetrasomy 12 was observed in 
      a case of ovarian thecoma.
FAU - Schofield, D E
AU  - Schofield DE
AD  - Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts.
FAU - Fletcher, J A
AU  - Fletcher JA
LA  - eng
GR  - 1K11CA01498-02/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Mucins)
SB  - IM
MH  - Cell Nucleus/ultrastructure
MH  - Child
MH  - Child, Preschool
MH  - *Chromosomes, Human, Pair 12
MH  - Female
MH  - Fluorescence
MH  - Granulosa Cell Tumor/chemistry/*genetics
MH  - Humans
MH  - In Situ Hybridization
MH  - Infant
MH  - Karyotyping
MH  - Male
MH  - Methods
MH  - Mucins/analysis
MH  - Ovarian Neoplasms/chemistry/*genetics
MH  - Testicular Neoplasms/chemistry/*genetics
MH  - *Trisomy
PMC - PMC1886775
EDAT- 1992/12/01 00:00
MHDA- 1992/12/01 00:01
PMCR- 1993/06/01
CRDT- 1992/12/01 00:00
PHST- 1992/12/01 00:00 [pubmed]
PHST- 1992/12/01 00:01 [medline]
PHST- 1992/12/01 00:00 [entrez]
PHST- 1993/06/01 00:00 [pmc-release]
PST - ppublish
SO  - Am J Pathol. 1992 Dec;141(6):1265-9.