PMID- 14665747
OWN - NLM
STAT- MEDLINE
DCOM- 20040206
LR  - 20190605
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 65
IP  - 11
DP  - 2003 Nov
TI  - Development of an efficient method for recovery of Puumala and Puumala-related 
      viruses by inoculation of Mongolian gerbils.
PG  - 1189-94
AB  - Puumala (PUU) virus and PUU-related viruses are difficult to isolate in cell 
      culture. To determine whether animal inoculation would be a better alternative 
      for virus recovery, the Sotkamo strain of PUU virus was inoculated into several 
      animal species. Newborn Mongolian gerbils (MGs), mice, and rats were infected 
      with the Sotkamo strain by intracerebral (ic), intraperitoneal (ip), and 
      subcutaneous (sc) inoculation. Antibodies to PUU appeared in MGs at 30 days 
      post-infection (dpi), and in mice and rats at 15 dpi. Interestingly, virus 
      appeared at 7 dpi in lung and brain of MGs inoculated via ic and ip routes. Virus 
      was detected in all tested tissues of MGs at 15 dpi, with a peak level of 1.36 x 
      10 (5) focus forming units (FFU)/g in brain tissue. The virus titer declined with 
      the onset of the antibody response and became undetectable by 75 dpi, when the 
      antibody titer reached the maximum level. The appearance of the virus in mice and 
      rats was delayed as compared to MGs, and the virus titer was apparently lower, at 
      approximately 4 to 8 x 10(3) FFU/g, at 15 dpi. In addition, lung homogenates of 
      antibody-positive Clethrionomys (C.) rufocanus (captured in Tobetsu, Hokkaido, 
      Japan) were inoculated into MGs by the ic route. PUU-related viral RNA was 
      detected at 16 dpi in the brains of MG inoculated with the lung homogenate, and 
      antibodies were detected at 45 dpi. These findings indicate that newborn MG 
      inoculation is an efficient method to recover PUU and PUU-related viruses.
FAU - Lokugamage, Nandadeva
AU  - Lokugamage N
AD  - Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
FAU - Kariwa, Hiroaki
AU  - Kariwa H
FAU - Lokugamage, Kumari
AU  - Lokugamage K
FAU - Hagiya, Tomohiro
AU  - Hagiya T
FAU - Miyamoto, Hironobu
AU  - Miyamoto H
FAU - Iwasa, Masahiro A
AU  - Iwasa MA
FAU - Araki, Koichi
AU  - Araki K
FAU - Yoshimatsu, Kumiko
AU  - Yoshimatsu K
FAU - Arikawa, Jiro
AU  - Arikawa J
FAU - Mizutani, Tetsuya
AU  - Mizutani T
FAU - Takashima, Ikuo
AU  - Takashima I
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (DNA Primers)
SB  - IM
MH  - Animals
MH  - Antibody Formation
MH  - DNA Primers
MH  - Fluorescent Antibody Technique, Indirect
MH  - Gerbillinae/*virology
MH  - Mice
MH  - Puumala virus/*growth & development/*isolation & purification
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Virus Cultivation
EDAT- 2003/12/11 05:00
MHDA- 2004/02/10 05:00
CRDT- 2003/12/11 05:00
PHST- 2003/12/11 05:00 [pubmed]
PHST- 2004/02/10 05:00 [medline]
PHST- 2003/12/11 05:00 [entrez]
AID - 10.1292/jvms.65.1189 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2003 Nov;65(11):1189-94. doi: 10.1292/jvms.65.1189.