PMID- 14665956
OWN - NLM
STAT- MEDLINE
DCOM- 20040129
LR  - 20061115
IS  - 0022-5347 (Print)
IS  - 0022-5347 (Linking)
VI  - 171
IP  - 1
DP  - 2004 Jan
TI  - Monocyte chemoattractant protein-1 production by human detrusor smooth muscle 
      cells.
PG  - 462-6
AB  - PURPOSE: Most recently attention has turned to the secretory properties of smooth 
      muscle cells. Monocyte chemoattractant protein-1 (MCP-1) is a potent chemokine 
      that causes mast cells recruitment and provokes mast cells activation in vitro. 
      We investigated whether MCP-1 is produced by human detrusor smooth muscle cells 
      (HDSMCs) cultured under inflammatory conditions. MATERIALS AND METHODS: Using an 
      explantation technique HDSMCs were isolated and short-term cultured. HDSMCs were 
      incubated at 37C for 24 hours with the proinflammatory mediators interleukin-1 
      beta (IL-1 beta), tumor necrosis factor (TNF-alpha), lipopolysaccharide, 
      histamine, leukotriene D4 and prostaglandin E2. The level of MCP-1 in cell 
      supernatants were measured by enzyme linked immunoassay. RESULTS: There was basal 
      secretion of MCP-1 in unstimulated cultures. Following 24-hour incubation with 
      IL-1 beta or TNF-alpha (1 pg/ml to 100 ng/ml) the level of MCP-1 increased in a 
      dose dependent manner. IL-1 beta was more potent at inducing MCP-1 release in 8 
      of 10 experiments. Lipopolysaccharide (10 microg/ml), histamine (100 microM), 
      leukotriene D4 (50 nM), prostaglandin E2 (1 microM) and KCl (30 to 100 mM) failed 
      to induce MCP-1 production. When IL-1 beta (10 ng/ml) and TNF-alpha (10 ng/ml) 
      were given in combination, a highly synergistic effect on MCP-1 production was 
      observed. CONCLUSIONS: To our knowledge this study shows for the first time that 
      human detrusor smooth muscle cells cultivated under inflammatory conditions 
      produce significant amounts of MCP-1. In addition to contractile function, HDSMCs 
      have synthesis and secretory potential with the release of MCP-1. Thus, MCP-1 may 
      contribute to the local inflammatory process by producing proinflammatory 
      mediators. The release of cytokines and chemokines by human detrusor muscle even 
      in small amounts may have important functional consequences.
FAU - Bouchelouche, Kirsten
AU  - Bouchelouche K
AD  - Smooth Mucle Laboratory and Department of Urology, Herlev Hospital, University of 
      Copenhagen, Denmark. kibou@herlevhosp.kbhamt.dk
FAU - Alvarez, Susana
AU  - Alvarez S
FAU - Andersen, Lisa
AU  - Andersen L
FAU - Nordling, Jorgen
AU  - Nordling J
FAU - Horn, Thomas
AU  - Horn T
FAU - Bouchelouche, Pierre
AU  - Bouchelouche P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Urol
JT  - The Journal of urology
JID - 0376374
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Chemokine CCL2/*biosynthesis
MH  - Humans
MH  - Muscle, Smooth/*cytology/*metabolism
MH  - Urinary Bladder/cytology
EDAT- 2003/12/11 05:00
MHDA- 2004/01/30 05:00
CRDT- 2003/12/11 05:00
PHST- 2003/12/11 05:00 [pubmed]
PHST- 2004/01/30 05:00 [medline]
PHST- 2003/12/11 05:00 [entrez]
AID - S0022-5347(05)62781-4 [pii]
AID - 10.1097/01.ju.0000090192.36436.d5 [doi]
PST - ppublish
SO  - J Urol. 2004 Jan;171(1):462-6. doi: 10.1097/01.ju.0000090192.36436.d5.