PMID- 14666671
OWN - NLM
STAT- MEDLINE
DCOM- 20040211
LR  - 20091119
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 23
IP  - 5A
DP  - 2003 Sep-Oct
TI  - Constitutive expression of Rb associated protein 46 (RbAp46) reverts transformed 
      phenotypes of breast cancer cells.
PG  - 3735-40
AB  - The retinoblastoma (Rb) suppressor associated protein 46 (RbAp46) is a subunit of 
      chromatin modifying and remodeling complexes. Previously, we found that RbAp46 
      functions as a potent growth inhibitor. It is also a downstream effector of the 
      Wilms' tumor suppressor, WT1. The findings that expression levels of WT1 were 
      down-regulated in breast cancer cell lines and in subsets of primary breast 
      tumors led us to investigate the possible role of RbAp46 in breast cancer 
      tumorigenesis. Here, we found that RbAp46 expression levels were decreased in 
      five established breast cancer cell lines compared to a normal mammary gland 
      epithelial cell line. To investigate the effect of constitutive expression of 
      RbAp46 on the transformed phenotypes of breast cancer cells, we established 
      stable cell lines that constitutively express exogenous RbAp46 using three breast 
      cancer cell lines, MCF-7, MDA-MB-231 and MDA-MB-436. We have found that RbAp46 
      expression suppressed colony formation of these breast cancer cells in soft-agar, 
      and inhibited tumor formation of these cells in nude mice. Our data demonstrated 
      that constitutive RbAp46 expression suppresses the transformed phenotypes of 
      breast cancer cells, and suggested that dysregulation of RbAp46 expression may 
      contribute to breast cancer tumorigenesis.
FAU - Zhang, Teng-Fei
AU  - Zhang TF
AD  - Creighton University Medical Center, 2500 California Plaza, Omaha, NE 68178, USA.
FAU - Yu, Shui-Qing
AU  - Yu SQ
FAU - Deuel, Thomas F
AU  - Deuel TF
FAU - Wang, Zhao-Yi
AU  - Wang ZY
LA  - eng
GR  - CA 84328/CA/NCI NIH HHS/United States
GR  - CA84400/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Carrier Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RBBP7 protein, human)
RN  - 0 (Rbbp7 protein, mouse)
RN  - 0 (Retinoblastoma-Binding Protein 7)
SB  - IM
MH  - Animals
MH  - Breast Neoplasms/genetics/metabolism/*pathology
MH  - Carrier Proteins/biosynthesis/genetics/*physiology
MH  - Cell Line, Tumor
MH  - Cell Transformation, Neoplastic/genetics/metabolism/*pathology
MH  - Down-Regulation
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Mice
MH  - Mice, Nude
MH  - Nuclear Proteins/biosynthesis/genetics/*physiology
MH  - Retinoblastoma-Binding Protein 7
MH  - Transfection
EDAT- 2003/12/12 05:00
MHDA- 2004/02/12 05:00
CRDT- 2003/12/12 05:00
PHST- 2003/12/12 05:00 [pubmed]
PHST- 2004/02/12 05:00 [medline]
PHST- 2003/12/12 05:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 2003 Sep-Oct;23(5A):3735-40.