PMID- 14666981
OWN - NLM
STAT- MEDLINE
DCOM- 20040204
LR  - 20191108
IS  - 0723-2020 (Print)
IS  - 0723-2020 (Linking)
VI  - 26
IP  - 4
DP  - 2003 Nov
TI  - Candidatus "Scalindua brodae", sp. nov., Candidatus "Scalindua wagneri", sp. 
      nov., two new species of anaerobic ammonium oxidizing bacteria.
PG  - 529-38
AB  - Anaerobic ammonium oxidation (anammox) is both a promising process in wastewater 
      treatment and a long overlooked microbial physiology that can contribute 
      significantly to biological nitrogen cycling in the world's oceans. Anammox is 
      mediated by a monophyletic group of bacteria that branches deeply in the 
      Planctomycetales. Here we describe a new genus and species of anaerobic ammonium 
      oxidizing planctomycetes, discovered in a wastewater treatment plant (wwtp) 
      treating landfill leachate in Pitsea, UK. The biomass from this wwtp showed high 
      anammox activity (5.0 +/- 0.5 nmol/mg protein/min) and produced hydrazine from 
      hydroxylamine, one of the unique features of anammox bacteria. Eight new 
      planctomycete 16S rRNA gene sequences were present in the 16S rRNA gene clone 
      library generated from the biomass. Four of these were affiliated to known 
      anammox 16S rRNA gene sequences, but branched much closer to the root of the 
      planctomycete line of descent. Fluorescence in situ hybridization (FISH) with 
      oligonucleotide probes specific for these new sequences showed that two species 
      (belonging to the same genus) together made up > 99% of the planctomycete 
      population which constituted 20% of the total microbial community. The 
      identification of these organisms as typical anammox bacteria was confirmed with 
      electron microscopy and lipid analysis. The new species, provisionally named 
      Candidatus "Scalindua brodae" and "Scalindua wagneri" considerably extend the 
      biodiversity of the anammox lineage on the 16S rRNA gene level, but otherwise 
      resemble known anammox bacteria. Simultaneously, another new species of the same 
      genus, Candidatus "Scalindua sorokinii", was detected in the water column of the 
      Black Sea, making this genus the most widespread of all anammox bacteria 
      described so far.
FAU - Schmid, Markus
AU  - Schmid M
AD  - Kluyver Laboratory for Biotechnology, Delft University of Technology, Delft, The 
      Netherlands.
FAU - Walsh, Kerry
AU  - Walsh K
FAU - Webb, Rick
AU  - Webb R
FAU - Rijpstra, W Irene C
AU  - Rijpstra WI
FAU - van de Pas-Schoonen, Katinka
AU  - van de Pas-Schoonen K
FAU - Verbruggen, Mark Jan
AU  - Verbruggen MJ
FAU - Hill, Thomas
AU  - Hill T
FAU - Moffett, Bruce
AU  - Moffett B
FAU - Fuerst, John
AU  - Fuerst J
FAU - Schouten, Stefan
AU  - Schouten S
FAU - Damste, Jaap S Sinninghe
AU  - Damste JS
FAU - Harris, James
AU  - Harris J
FAU - Shaw, Phil
AU  - Shaw P
FAU - Jetten, Mike
AU  - Jetten M
FAU - Strous, Marc
AU  - Strous M
LA  - eng
SI  - GENBANK/AY254882
SI  - GENBANK/AY254883
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Syst Appl Microbiol
JT  - Systematic and applied microbiology
JID - 8306133
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Hydrazines)
RN  - 0 (Lipids)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 142M471B3J (Carbon Dioxide)
RN  - 27RFH0GB4R (hydrazine)
RN  - 2FP81O2L9Z (Hydroxylamine)
RN  - M0KG633D4F (Sodium Nitrite)
SB  - IM
MH  - Anaerobiosis
MH  - Bacteria, Anaerobic/*classification/cytology/*isolation & purification/physiology
MH  - Biofilms
MH  - Carbon Dioxide/*metabolism
MH  - DNA, Bacterial/chemistry/isolation & purification
MH  - DNA, Ribosomal/chemistry/isolation & purification
MH  - Genes, rRNA
MH  - Hydrazines/metabolism
MH  - Hydroxylamine/metabolism
MH  - In Situ Hybridization
MH  - Lipids/analysis/chemistry
MH  - Molecular Sequence Data
MH  - Oxidation-Reduction
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sodium Nitrite/*metabolism
MH  - United Kingdom
MH  - Water Microbiology
EDAT- 2003/12/12 05:00
MHDA- 2004/02/05 05:00
CRDT- 2003/12/12 05:00
PHST- 2003/12/12 05:00 [pubmed]
PHST- 2004/02/05 05:00 [medline]
PHST- 2003/12/12 05:00 [entrez]
AID - 10.1078/072320203770865837 [doi]
PST - ppublish
SO  - Syst Appl Microbiol. 2003 Nov;26(4):529-38. doi: 10.1078/072320203770865837.