PMID- 14667029
OWN - NLM
STAT- MEDLINE
DCOM- 20040224
LR  - 20061115
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 56
IP  - 3
DP  - 2003 Oct 24
TI  - Haematological analyses in rainbow trout Oncorhynchus mykiss affected by viral 
      haemorrhagic septicaemia (VHS).
PG  - 185-93
AB  - Rainbow trout Oncorhynchus mykiss weighing 87 +/- 15 g (mean +/- SD) were 
      infected with viral haemorrhagic septicaemia virus (VHSV) and the haematological 
      and biochemical profiles of peripheral blood examined. Depending on the clinical 
      signs and gross pathology, the fish were divided into 2 groups: Group A included 
      fish in the acute stage, Group B comprised fish in the chronic stage. Red blood 
      cells were subjected to 6 haematological tests and blood plasma to 14 biochemical 
      tests, which provided findings on changed substrate concentrations and enzyme 
      activities. Diseased fish, compared to healthy fish, had a significantly lower 
      red blood cell count, and lower haematocrit and haemoglobin levels. As for the 
      biochemical parameters, the fish had less total protein, creatinine, glucose, 
      triacylglycerol, inorganic phosphate, total calcium and sodium, and more blood 
      urea, nitrogen and potassium. Uric acid levels remained unchanged. Increases were 
      recorded in the catalytic concentration of alanine aminotransferase, aspartate 
      aminotransferase and lactate dehydrogenase. A decrease was recorded in the 
      catalytic concentration of alkaline phosphatase. Fish with VHS in the chronic 
      stage, compared with healthy fish, were in worse condition, with a significantly 
      reduced Fulton coefficient and Clark coefficient, and a higher hepatosomatic 
      index and visceral somatic index.
FAU - Rehulka, J
AU  - Rehulka J
AD  - Department of Zoology, Silesian Museum, Tyrsova 1, 746 46 Opava, Czech Republic. 
      jiri.rehulka@szmo.cz
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
RN  - 0 (Hemoglobins)
SB  - IM
MH  - Animals
MH  - Aquaculture
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Erythrocyte Count/veterinary
MH  - Fish Diseases/*blood/pathology
MH  - Hematocrit/veterinary
MH  - Hemoglobins
MH  - Hemorrhagic Septicemia, Viral/*blood/pathology
MH  - *Novirhabdovirus
MH  - Oncorhynchus mykiss/blood/*virology
MH  - Rhabdoviridae Infections/*veterinary
EDAT- 2003/12/12 05:00
MHDA- 2004/02/26 05:00
CRDT- 2003/12/12 05:00
PHST- 2003/12/12 05:00 [pubmed]
PHST- 2004/02/26 05:00 [medline]
PHST- 2003/12/12 05:00 [entrez]
AID - 10.3354/dao056185 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2003 Oct 24;56(3):185-93. doi: 10.3354/dao056185.