PMID- 14668208
OWN - NLM
STAT- MEDLINE
DCOM- 20041102
LR  - 20131121
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 70
IP  - 4
DP  - 2004 Apr
TI  - Expression and localization of hypoxia-inducible factor-1 subunits in the adult 
      rat epididymis.
PG  - 1121-30
AB  - The epididymal epithelium contributes to formation of a luminal fluid that is 
      essential for the protection of spermatozoa from a variety of insults including 
      changes in oxygen tension. A key regulator of the response to oxygen debt in many 
      cells is hypoxia-inducible factor-1 (HIF-1). A transcription factor composed of 
      alpha and beta subunits, HIF-1 activates genes that mediate oxygen homeostasis 
      and cell survival pathways or trigger cell death responses. Previously we have 
      shown that HIF-1alpha mRNA is expressed in the adult rat epididymis. Goals of 
      this study were to determine whether HIF-1alpha protein is activated by ischemia 
      in the rat epididymis, to determine whether epididymal HIF-1alpha mRNA expression 
      is androgen dependent, and to identify epididymal cell types expressing 
      HIF-1alpha and beta. Immunoblot analysis revealed that HIF-1alpha protein is 
      primarily present in corpus and cauda of the normoxic epididymis and unaffected 
      by ischemia, whereas HIF-1beta was detected equally in all regions and also 
      unaffected by ischemia. HIF-1alpha mRNA expression in all regions was not 
      affected by 15 days bilateral orchiectomy. Principal cells stained positive for 
      HIF-1alpha by immunocytochemistry, with the epithelium of initial segment and 
      caput epididymidis staining less intensely than corpus and cauda. HIF-1beta 
      immunoreactivity was equally present in principal cells in all regions. Clear, 
      narrow, and basal cells were unreactive for HIF-1alpha and beta. The presence of 
      HIF-1 in normoxic epididymis and the regional distribution of HIF-1alpha suggests 
      fundamental differences in how proximal and distal regions of the epididymis 
      maintain oxygen homeostasis to protect the epithelium and spermatozoa from 
      hypoxia.
FAU - Palladino, M A
AU  - Palladino MA
AD  - Department of Biology, Monmouth University, West Long Branch, New Jersey 07764, 
      USA. mpalladi@monmouth.edu
FAU - Powell, J D
AU  - Powell JD
FAU - Korah, N
AU  - Korah N
FAU - Hermo, L
AU  - Hermo L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20031210
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (ARNT protein, rat)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - 0 (Transcription Factors)
RN  - 138391-32-9 (Aryl Hydrocarbon Receptor Nuclear Translocator)
RN  - 3XMK78S47O (Testosterone)
SB  - IM
MH  - Animals
MH  - Aryl Hydrocarbon Receptor Nuclear Translocator
MH  - DNA-Binding Proteins/*metabolism
MH  - Epididymis/blood supply/*metabolism
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Ischemia/metabolism
MH  - Male
MH  - Orchiectomy
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Aryl Hydrocarbon/*metabolism
MH  - Spermatozoa/metabolism
MH  - Testosterone/blood/pharmacology
MH  - Tissue Distribution
MH  - Transcription Factors/genetics/*metabolism
EDAT- 2003/12/12 05:00
MHDA- 2004/11/04 09:00
CRDT- 2003/12/12 05:00
PHST- 2003/12/12 05:00 [pubmed]
PHST- 2004/11/04 09:00 [medline]
PHST- 2003/12/12 05:00 [entrez]
AID - biolreprod.103.023085 [pii]
AID - 10.1095/biolreprod.103.023085 [doi]
PST - ppublish
SO  - Biol Reprod. 2004 Apr;70(4):1121-30. doi: 10.1095/biolreprod.103.023085. Epub 
      2003 Dec 10.