PMID- 14669212
OWN - NLM
STAT- MEDLINE
DCOM- 20040415
LR  - 20071115
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 20
IP  - 6
DP  - 2003 Dec
TI  - [Molecular cytogenetic analysis of -7/7q- abnormalities in patients with myeloid 
      malignancies].
PG  - 471-6
AB  - OBJECTIVE: To accurately evaluate the incidence of -7/7q- abnormality in acute 
      myeloblastic leukemia (AML) and myelodysplastic syndrome (MDS) patients and 
      investigate the value of fluorescence in situ hybridization (FISH) technique in 
      the detection and identification of -7 and 7q abnormality. METHODS: A FISH assay 
      was performed to analyze 70 AML/MDS patients who had received conventional 
      cytogenetic analysis (CCA). The dual color probes CEP 7 labeled by SpectrumGreen 
      and D7S486 (locus at 7q31) labeled by SpectrumOrange were used. RESULTS: The 
      incidence of -7/7q- in AML and MDS patients was 4.51% (31 out of 687 cases) and 
      5.71% (28 out of 490 cases), respectively, and was 5.68% and 10.29% in these 
      patients with abnormal karyotype, respectively. The common deletion region of 7q- 
      was 7q21a222 (ten cases) and 7q31-35(ten cases). FISH assay confirmed the -7/7q- 
      aberration in those with clonal -7/7q- abnormalities, but failed in those with 
      random -7/7q- and normal karyotype. In 7q- group, FISH revealed seven of eleven 
      cases with monosomy 7 clone detected in the same specimen, but the numbers of 7q- 
      interphases cells were much greater than those of monosomy 7 cells (average 42.5% 
      vs 8.4%, P=0.025). FISH also provided precise refinement for three chromosomal 
      structural abnormalities associated with 7q seen in CAA, one case with 
      del(7)(q22) being refined as chromosomal translocation, one case with 7q+ being 
      confirmed as dup(7q), and one case with complex translocation involving 7q being 
      also proved to be true. CONCLUSION: FISH is a powerful tool to identify or refine 
      chromosomal structural aberrations involving 7q, and it provides accurate 
      evaluation of -7/7q- in all the patients. -7 and 7q- clone frequently coexist in 
      the same specimen, and the significantly increasing percentage of 7q- cells 
      implies that -7 clone secondary to 7q- clone is a result from loss of 7q-.
FAU - Xiao, Yun
AU  - Xiao Y
AD  - Department of Clinical Hematological Examinations, Affiliated Hospital of Guiyang 
      Medical College, Guiyang, Guizhou, 550001 PR China.
FAU - Liu, Shi-he
AU  - Liu SH
FAU - Liu, Xu-ping
AU  - Liu XP
FAU - Qin, Shuang
AU  - Qin S
FAU - Bo, Li-jin
AU  - Bo LJ
FAU - Li, Cheng-wen
AU  - Li CW
FAU - Dai, Yun
AU  - Dai Y
FAU - Wang, Ji-shi
AU  - Wang JS
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Adult
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 7
MH  - Cytogenetic Analysis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myeloid, Acute/*genetics
MH  - Male
MH  - Myelodysplastic Syndromes/*genetics
EDAT- 2003/12/12 05:00
MHDA- 2004/04/16 05:00
CRDT- 2003/12/12 05:00
PHST- 2003/12/12 05:00 [pubmed]
PHST- 2004/04/16 05:00 [medline]
PHST- 2003/12/12 05:00 [entrez]
AID - 940620126 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2003 Dec;20(6):471-6.