PMID- 14670321
OWN - NLM
STAT- MEDLINE
DCOM- 20040319
LR  - 20190728
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 22
IP  - 3-4
DP  - 2004 Jan 2
TI  - Induction of persistent in vivo resistance to Mycobacterium avium infection in 
      BALB/c mice injected with interleukin-18-secreting fibroblasts.
PG  - 398-406
AB  - Interferon-gamma (IFN-gamma) is closely associated with the generation of 
      cell-mediated immunity and resistance to intracellular parasites. Interleukin-18 
      (IL-18) is known to strongly induce IFN-gamma production by T cells and natural 
      killer (NK) cells. To determine whether the paracrine secretion of IL-18 can 
      efficiently stimulate the resistance to Mycobacterium avium complex (MAC) 
      infection, 3T3 fibroblasts were stably transfected to secrete bioactive IL-18 and 
      their effects on MAC infection were investigated in genetically susceptible 
      BALB/c mice, compared with that of free recombinant IL-18. Immunization with 
      IL-18-secreting fibroblasts (3T3/IL-18) during intranasal infection with MAC 
      resulted in a significant decrease in bacterial load of lung during the entire 
      8-week observation period, while rIL-18 reduced the bacterial load at initial 1 
      week but not by 8 weeks postinfection. Immunization with the 3T3/IL-18 cells 
      induced and maintained significantly higher levels of cytotoxic activity and 
      nitric oxide production by lung cells than those of rIL-18 immunization. 
      Furthermore, lung cells in mice injected with the 3T3/IL-18 cells showed 
      persistent production of IFN-gamma throughout the 8-week period, suggesting that 
      the 3T3/IL-18 cells induced the resistance to MAC infection via IFN-gamma 
      production. This work suggests that IL-18-secreting fibroblasts may serve as a 
      vehicle for paracrine secretion of IL-18 in immunotherapy of MAC infection.
FAU - Chung, Su W
AU  - Chung SW
AD  - Department of Pharmacy, College of Pharmacy, Chonnam National University, 500-757 
      Kwangju, Republic of Korea.
FAU - Choi, Sang H
AU  - Choi SH
FAU - Kim, Tae S
AU  - Kim TS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Cytokines)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Interleukin-18)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Antibodies, Monoclonal/biosynthesis/pharmacology
MH  - Blotting, Western
MH  - Cytokines/biosynthesis
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Female
MH  - Fibroblasts/*metabolism
MH  - Genetic Vectors/genetics
MH  - Indicators and Reagents
MH  - Interferon-gamma/immunology
MH  - Interleukin-18/*metabolism
MH  - Lung/cytology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mycobacterium avium Complex/*immunology
MH  - Mycobacterium avium-intracellulare Infection/*immunology/microbiology/*prevention 
      & control
MH  - Nitric Oxide/metabolism
MH  - Retroviridae/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - T-Lymphocyte Subsets/immunology
MH  - Transfection
EDAT- 2003/12/13 05:00
MHDA- 2004/03/20 05:00
CRDT- 2003/12/13 05:00
PHST- 2003/12/13 05:00 [pubmed]
PHST- 2004/03/20 05:00 [medline]
PHST- 2003/12/13 05:00 [entrez]
AID - S0264410X0300584X [pii]
AID - 10.1016/j.vaccine.2003.07.002 [doi]
PST - ppublish
SO  - Vaccine. 2004 Jan 2;22(3-4):398-406. doi: 10.1016/j.vaccine.2003.07.002.