PMID- 14670329
OWN - NLM
STAT- MEDLINE
DCOM- 20040319
LR  - 20190728
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 22
IP  - 3-4
DP  - 2004 Jan 2
TI  - Immune response of pigs inoculated with Mycobacterium bovis BCG expressing a 
      truncated form of GP5 and M protein of porcine reproductive and respiratory 
      syndrome virus.
PG  - 467-74
AB  - Pigs were immunised with recombinant BCG (rBCG) expressing a truncated form of 
      GP5 (lacking the first 30 NH(2)-terminal residues) (rBCGGP5) and M protein 
      (rBCGM) of porcine reproductive and respiratory syndrome virus (PRRSV). At 30 
      days post-inoculation (dpi), pigs inoculated with rBCGGP5 and rBCGM developed a 
      specific humoral immune response against the viral proteins, as detected by 
      commercial ELISA and Western blot tests, and at 60 dpi, three out of five animals 
      developed neutralizing antibodies with titers ranging from 1:4 to 1:8. At 67 dpi, 
      an IFN-gamma response against BCG antigens, but not against the viral proteins, 
      was detected by ELISPOT in inoculated pigs. Following challenge with a pathogenic 
      strain of PRRSV, pigs inoculated with rBCG showed lower (P<0.05) temperature, 
      viremia and virus load in bronchial lymph nodes than control animals, suggesting 
      the establishment of partial protection against PRRSV infection.
FAU - Bastos, Reginaldo G
AU  - Bastos RG
AD  - Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, 
      Lincoln, NE, USA.
FAU - Dellagostin, Odir A
AU  - Dellagostin OA
FAU - Barletta, Raul G
AU  - Barletta RG
FAU - Doster, Allan R
AU  - Doster AR
FAU - Nelson, Eric
AU  - Nelson E
FAU - Zuckermann, Federico
AU  - Zuckermann F
FAU - Osorio, Fernando A
AU  - Osorio FA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antibodies, Viral)
RN  - 0 (BCG Vaccine)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Adjuvants, Immunologic/*pharmacology
MH  - Animals
MH  - Antibodies, Viral/*biosynthesis
MH  - Antibody Formation/immunology
MH  - BCG Vaccine/chemistry/*immunology
MH  - Blotting, Western
MH  - Body Temperature
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunity, Cellular/immunology
MH  - Lymph Nodes/virology
MH  - Palatine Tonsil/virology
MH  - Porcine Reproductive and Respiratory Syndrome/blood/*immunology/*prevention & 
      control
MH  - Porcine respiratory and reproductive syndrome virus/chemistry/*immunology
MH  - Swine
MH  - Viral Envelope Proteins/biosynthesis/*genetics
MH  - Viral Load
MH  - Viral Proteins/*immunology
EDAT- 2003/12/13 05:00
MHDA- 2004/03/20 05:00
CRDT- 2003/12/13 05:00
PHST- 2003/12/13 05:00 [pubmed]
PHST- 2004/03/20 05:00 [medline]
PHST- 2003/12/13 05:00 [entrez]
AID - S0264410X03005723 [pii]
AID - 10.1016/s0264-410x(03)00572-3 [doi]
PST - ppublish
SO  - Vaccine. 2004 Jan 2;22(3-4):467-74. doi: 10.1016/s0264-410x(03)00572-3.