PMID- 14672282
OWN - NLM
STAT- MEDLINE
DCOM- 20040128
LR  - 20190402
IS  - 0304-4920 (Print)
IS  - 0304-4920 (Linking)
VI  - 46
IP  - 3
DP  - 2003 Sep 30
TI  - Homocysteine altered ROS generation and NO accumulation in endothelial cells.
PG  - 129-36
AB  - Mild hyperhomocysteinemia (HHcy) is a risk factor for vascular disease and is 
      closely associated with endothelial dysfunction. Oxidative stress and decreased 
      nitric oxide (NO) bioavailability were reported in HHcy-induced vascular injury; 
      however, the exact relationship is not understood. We thus directly determine the 
      production of reactive oxygen species (ROS) and NO in cultured endothelial cells 
      (HUVECs) to demonstrate the correlated variation between ROS and NO induced by 
      Hcy (homocysteine), Cys (cysteine), another thiol compound, and Met (methionine), 
      precursor of HHcy in animal study. HUVECs were treated with Hcy, Cys, or Met for 
      0.5 or 22-24 h; ROS generation was detected by DCF fluorescence with flow 
      cytometry and NO by chemiluminescence. In non-cytotoxic (<1.0 mM) concentration 
      ranges, Met exerted no effects on either ROS production or NO concentration, Cys 
      decreased ROS production and increased NO in both short-term (0.5 h) and 
      long-term (22-24 h) treatments; Hcy, however, induced a biphasic effect on ROS 
      production, i.e., inhibitory at 0.5 h but stimulatory at 24 h. The maximal 
      stimulation by Hcy (0.25 mM) was significantly reduced by co-incubation (12 h) 
      with estrogen (1 microM). Hcy caused an early (0.5 h) increase of medium NO which 
      was absent in long-term Hcy treatment. The oxidative stress caused by long-term 
      Hcy incubation could be ameliorated by estrogen, consistent with earlier in vivo 
      observations that estrogen prevents HHcy-induced injury.
FAU - Tsen, Chih-Mei
AU  - Tsen CM
AD  - Department of Physiology and Pharmacology, Chang Gung University College of 
      Medicine, Taoyuan, Taiwan, ROC.
FAU - Hsieh, Chien-Cheng
AU  - Hsieh CC
FAU - Yen, Chia-Hung
AU  - Yen CH
FAU - Lau, Ying-Tung
AU  - Lau YT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Chin J Physiol
JT  - The Chinese journal of physiology
JID - 7804502
RN  - 0 (Reactive Oxygen Species)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - AE28F7PNPL (Methionine)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Cysteine/pharmacology
MH  - Endothelium, Vascular/cytology/*drug effects/*metabolism
MH  - Homocysteine/*pharmacology
MH  - Humans
MH  - Methionine/pharmacology
MH  - Nitric Oxide/*metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Umbilical Veins/cytology
EDAT- 2003/12/16 05:00
MHDA- 2004/01/30 05:00
CRDT- 2003/12/16 05:00
PHST- 2003/12/16 05:00 [pubmed]
PHST- 2004/01/30 05:00 [medline]
PHST- 2003/12/16 05:00 [entrez]
PST - ppublish
SO  - Chin J Physiol. 2003 Sep 30;46(3):129-36.