PMID- 14672336
OWN - NLM
STAT- MEDLINE
DCOM- 20040126
LR  - 20190922
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 50
IP  - 4
DP  - 2003 Oct
TI  - Down-regulation of HLA-G1 cell surface expression in human cytomegalovirus 
      infected cells.
PG  - 328-33
AB  - PROBLEM: Down-modulation of human leukocyte antigen (HLA)-G1 cell surface 
      expression by human cytomegalovirus (HCMV) has only been studied in cellular 
      models expressing independent unique short (US) recombinant proteins, but not in 
      the context of viral infection. To explore the level of HLA-G1 cell surface 
      expression after HCMV infection and to investigate the influence of US viral 
      proteins, we infected HLA-G1 expressing cells by HCMV laboratory strains. METHOD 
      OF STUDY: Human U373-MG astrocytoma cells were transfected with HLA-G1 cDNA. 
      Following HCMV infection, HLA-G1 cell surface expression of these transfectants 
      was evaluated by flow cytometry and confocal microscopy, using an HLA-G specific 
      monoclonal antibody, and compared with that of uninfected cells. US-deleted 
      viruses were then used to evaluate the implication of US proteins. RESULTS: Using 
      flow cytometry, it was found that HCMV infection of U373-G1 cells decreased 
      HLA-G1 cell surface expression. Similar results were obtained with two different 
      HCMV strains, namely Towne and AD169. Two color confocal microscopy staining 
      further confirmed such HLA-G down-modulation in HCMV-infected cells stained for 
      immediate early (IE1/2) nuclear proteins expression. Infection of U373-G1 cells 
      with US-deleted HCMV strain had no effect on the level of cell surface HLA-G1 
      expression, thus demonstrating the US dependency of the HCMV-mediated 
      down-regulation of HLA-G1. CONCLUSION: HCMV infection down-modulates HLA-G1 
      expression at the cell surface. This is likely to have functional consequences in 
      case of HCMV uterine infection during pregnancy.
FAU - Pizzato, Nathalie
AU  - Pizzato N
AD  - INSERM U563, Centre de Physiopathologie Toulouse-Purpan, Toulouse, France.
FAU - Garmy-Susini, Barbara
AU  - Garmy-Susini B
FAU - Le Bouteiller, Philippe
AU  - Le Bouteiller P
FAU - Lenfant, Francoise
AU  - Lenfant F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Cell Line
MH  - Cell Membrane/immunology
MH  - Cytomegalovirus/genetics/immunology/pathogenicity
MH  - Cytomegalovirus Infections/genetics/*immunology
MH  - Down-Regulation
MH  - Gene Deletion
MH  - Genes, Viral
MH  - HLA Antigens/genetics/*metabolism
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/genetics/*metabolism
MH  - Humans
MH  - Models, Biological
MH  - Transfection
MH  - Viral Proteins/genetics
EDAT- 2003/12/16 05:00
MHDA- 2004/01/27 05:00
CRDT- 2003/12/16 05:00
PHST- 2003/12/16 05:00 [pubmed]
PHST- 2004/01/27 05:00 [medline]
PHST- 2003/12/16 05:00 [entrez]
AID - 10.1034/j.1600-0897.2003.00087.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2003 Oct;50(4):328-33. doi: 
      10.1034/j.1600-0897.2003.00087.x.