PMID- 14672338
OWN - NLM
STAT- MEDLINE
DCOM- 20040126
LR  - 20190922
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 50
IP  - 4
DP  - 2003 Oct
TI  - Increasing circulating T-cell activation markers are linked to subsequent 
      implantation failure after transfer of in vitro fertilized embryos.
PG  - 340-5
AB  - PROBLEM: Implantation determines success of in vitro fertilization (IVF) and 
      embryo transfer (ET) cycles. Data are accumulating to support a role of the 
      immune system in implantation. Most of the literature addresses the importance of 
      natural killer (NK) cells in this process. The purpose of the current study is to 
      examine the role of circulating T cells in implantation failure. METHOD OF STUDY: 
      Blood from 22 women undergoing IVF/ET during November, 2001, was drawn on cycle 
      day 9 and analyzed for the percentage of circulating T cells expressing the 
      activation markers CD69+ and human leukocyte antigen (HLA)-DR and the suppressor 
      marker CD11b using immunofluorescence and flow cytometry. These results were 
      compared with total percentage circulating CD3, CD4 and CD8 cells as well as NK 
      cells and pregnancy outcome that cycle. RESULTS: Infertile women had 
      significantly greater expression of the activation marker of CD69+ among CD8+ and 
      CD4+ T cells and HLA-DR among CD4 cells than fertile women. No difference in 
      expression of T cell suppressor marker of CD11b was noted when infertile and 
      fertile women were compared. No correlations were observed when activated T cells 
      were compared with circulating CD3+, CD4+, CD8+, activated NK cells and NK 
      cytotoxicity. CD3+ 4+ HLA-DR+ was expressed significantly less among successfully 
      pregnant compared with unsuccessfully pregnant women. CONCLUSION: T-cell 
      activation markers CD 69+ and HLA-DR+ are associated with increased implantation 
      failure after IVF/ET.
FAU - Coulam, Carolyn B
AU  - Coulam CB
AD  - Sher Institute for Reproductive Medicine, Chicago, IL, USA. cbcoulam@aol.com
FAU - Roussev, Roumen G
AU  - Roussev RG
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (Biomarkers)
RN  - 0 (CD69 antigen)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Lectins, C-Type)
SB  - IM
MH  - Adult
MH  - Antigens, CD/blood
MH  - Antigens, Differentiation, T-Lymphocyte/blood
MH  - Biomarkers/blood
MH  - Case-Control Studies
MH  - Cytotoxicity, Immunologic
MH  - Embryo Implantation/*immunology
MH  - *Embryo Transfer
MH  - Female
MH  - Fertilization in Vitro
MH  - HLA-DR Antigens/blood
MH  - Humans
MH  - Killer Cells, Natural/immunology
MH  - Lectins, C-Type
MH  - Lymphocyte Activation
MH  - Pregnancy
MH  - Pregnancy Outcome
MH  - T-Lymphocyte Subsets/immunology
MH  - T-Lymphocytes/*immunology
EDAT- 2003/12/16 05:00
MHDA- 2004/01/27 05:00
CRDT- 2003/12/16 05:00
PHST- 2003/12/16 05:00 [pubmed]
PHST- 2004/01/27 05:00 [medline]
PHST- 2003/12/16 05:00 [entrez]
AID - 10.1034/j.1600-0897.2003.00090.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2003 Oct;50(4):340-5. doi: 10.1034/j.1600-0897.2003.00090.x.