PMID- 14673014
OWN - NLM
STAT- MEDLINE
DCOM- 20040423
LR  - 20061115
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 75
IP  - 3
DP  - 2004 Mar
TI  - In situ demonstration of dendritic cell migration from rat intestine to 
      mesenteric lymph nodes: relationships to maturation and role of chemokines.
PG  - 434-42
AB  - Dendritic cells (DCs) are continuously transported from the intestine to 
      mesenteric lymph nodes (MLNs). The objective of this study was to determine the 
      migration kinetics of DCs via intestinal lymph and to investigate regulatory 
      factors affecting their migration in vivo. DCs were obtained from spleen or 
      thoracic duct lymph of mesenteric lymphadenectomized rats. The DCs were 
      fluorescently labeled and injected into the subserosa of the small intestine near 
      the cecum, and their migration patterns into MLNs were determined. Isolated DCs 
      from intestinal lymph express intercellular adhesion molecule-1 (ICAM-1), 
      CD11b/c, CD80/86, and major histocompatibility complex class II but maintain 
      their ability to phagocytize latex particles, suggesting the presence of immature 
      DCs. The isolated DCs accumulated in MLNs in a time-dependent manner with maximal 
      accumulation at 48 h. Cytokine-induced maturation of lymph DCs did not cause a 
      change in cell number but accelerated their transport into MLNs with a maximum at 
      24 h. Splenic DCs showed an intermediate level of maturation and a migration 
      pattern similar to mature DCs. Inhibition of ICAM-1 or CD11b/c did not affect DC 
      migration. Migration of mature DCs to MLNs was specifically blocked by 
      desensitization of CCR7 with CCL21. In contrast, freshly isolated lymph DCs were 
      not chemotactic for CCL21, but their migration to MLNs was mainly inhibited by 
      desensitization of CCR6 with CCL20. The migratory ability of DCs correlates well 
      with their degree of maturation, and different chemokine/chemokine receptor use 
      may be the main regulator of DC migration kinetics through intestinal lymph.
FAU - Kobayashi, Hisashi
AU  - Kobayashi H
AD  - Department of Internal Medicine, Keio University, School of Medicine, Tokyo, 
      Japan.
FAU - Miura, Soichiro
AU  - Miura S
FAU - Nagata, Hiroshi
AU  - Nagata H
FAU - Tsuzuki, Yoshikazu
AU  - Tsuzuki Y
FAU - Hokari, Ryota
AU  - Hokari R
FAU - Ogino, Takashi
AU  - Ogino T
FAU - Watanabe, Chikako
AU  - Watanabe C
FAU - Azuma, Toshifumi
AU  - Azuma T
FAU - Ishii, Hiromasa
AU  - Ishii H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20031212
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Antigens, Surface)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chemokines)
RN  - 0 (Chemokines, CC)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Animals
MH  - Antigens, Surface/analysis
MH  - Cell Adhesion Molecules/physiology
MH  - Chemokines/physiology
MH  - Chemokines, CC/physiology
MH  - *Chemotaxis
MH  - Dendritic Cells/chemistry/*cytology/immunology
MH  - Intestines/*cytology
MH  - Kinetics
MH  - Lymph Nodes/*cytology
MH  - Male
MH  - Mesentery/cytology
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Chemokine/physiology
EDAT- 2003/12/16 05:00
MHDA- 2004/04/24 05:00
CRDT- 2003/12/16 05:00
PHST- 2003/12/16 05:00 [pubmed]
PHST- 2004/04/24 05:00 [medline]
PHST- 2003/12/16 05:00 [entrez]
AID - jlb.0603250 [pii]
AID - 10.1189/jlb.0603250 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2004 Mar;75(3):434-42. doi: 10.1189/jlb.0603250. Epub 2003 Dec 12.