PMID- 14674023
OWN - NLM
STAT- MEDLINE
DCOM- 20040823
LR  - 20191108
IS  - 1009-3095 (Print)
IS  - 1009-3095 (Linking)
VI  - 5
IP  - 2
DP  - 2004 Feb
TI  - Programmed cell death features in apple suspension cells under low oxygen 
      culture.
PG  - 137-43
AB  - Suspension-cultured apple fruit cells (Malus pumila Mill. cv. Braeburn) were 
      exposed to a low oxygen atmosphere to test whether programmed cell death (PCD) 
      has a role in cell dysfunction and death under hypoxic conditions. Protoplasts 
      were prepared at various times after low oxygen conditions were established, and 
      viability tested by triple staining with fluorescein diacetate (FDA), propidium 
      iodide (PI) and Hoechst33342 (HO342). DNA breakdown and phosphatidylserine 
      exposure on the plasma membrane were observed using terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL), and annexin V binding. About 
      30% of protoplasts from cells after 48 h under low oxygen showed an increased 
      accumulation of HO342, indicating increased membrane permeability. Positive TUNEL 
      and annexin V results were also only obtained with protoplasts from cells under 
      low oxygen. The results suggest that apple cell death under low oxygen is at 
      least partially PCD mediated, and may explain tissue breakdown under controlled 
      atmosphere (low oxygen) conditions in apple fruit.
FAU - Xu, Chang-jie
AU  - Xu CJ
AD  - Laboratory of Fruit Molecular Physiology and Biotechnology/The State Agriculture 
      Ministry Laboratory of Horticultural Plant Growth, Development & Biotechnology, 
      Zhejiang University, Hangzhou 310029, China. chjxu@zju.edu.cn
FAU - Chen, Kun-song
AU  - Chen KS
FAU - Ferguson, Ian B
AU  - Ferguson IB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - J Zhejiang Univ Sci
JT  - Journal of Zhejiang University. Science
JID - 100954270
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Adaptation, Physiological/physiology
MH  - Apoptosis/*physiology
MH  - Cell Hypoxia/*physiology
MH  - Cell Survival/physiology
MH  - Cells, Cultured
MH  - DNA Damage/physiology
MH  - Fruit/*physiology
MH  - Malus/*physiology
MH  - Oxygen/*metabolism
EDAT- 2003/12/16 05:00
MHDA- 2004/08/24 05:00
CRDT- 2003/12/16 05:00
PHST- 2003/12/16 05:00 [pubmed]
PHST- 2004/08/24 05:00 [medline]
PHST- 2003/12/16 05:00 [entrez]
AID - 10.1007/BF02840914 [doi]
PST - ppublish
SO  - J Zhejiang Univ Sci. 2004 Feb;5(2):137-43. doi: 10.1007/BF02840914.