PMID- 14675414
OWN - NLM
STAT- MEDLINE
DCOM- 20040202
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 124
IP  - 1
DP  - 2004 Jan
TI  - Clinical relevance of balance between type 1 and type 2 immune responses of 
      lymphocyte subpopulations in aplastic anaemia patients.
PG  - 97-105
AB  - Immune dysfunction, which leads to the suppression of haemopoiesis by cytokines 
      that are secreted by activated T lymphocytes, is considered to play a key role in 
      the pathogenesis of acquired aplastic anaemia (AAA). We investigated the 
      intracytoplasmic expression of type-1 [interferon gamma (IFN-gamma), interleukin 
      (IL)-2] and type-2 (IL-4, IL-10) cytokines in CD4+ and CD8+ T cells before and 
      after in vitro activation in 16 patients with AAA and 17 normal controls. 
      Untreated or refractory patients had a significantly higher proportion of 
      unstimulated CD4+ and CD8+ T cells that produced IFN-gamma and IL-2 whereas the 
      IL-4 and IL-10 producing T cells did not differ from that of controls, resulting 
      in a shift of IFN-gamma/IL-4 ratio towards a type-1 response. Patients in 
      remission had also increased proportion of IFN-gamma-producing unstimulated CD4+ 
      and CD8+ cells, with a parallel rise of IL-4- and IL-10-producing cells and 
      normal IFN-gamma/IL-4 ratio. These data indicate that, in newly diagnosed and 
      refractory patients with AAA, CD4+ cells are polarized towards a type-1 response 
      that in turn leads to activation of cytotoxic CD8+ cells and finally to 
      haemopoietic stem cell destruction. The type-1 response persists in patients in 
      remission although this effect is compensated by the increase of IL-4 and IL-10 
      production.
FAU - Giannakoulas, Nikolaos C
AU  - Giannakoulas NC
AD  - Haematology Division, Department of Internal Medicine, University Hospital of 
      Patras, Rion, Patras, Greece. ngiannakoulas@hotmail.com
FAU - Karakantza, Marina
AU  - Karakantza M
FAU - Theodorou, Georgios L
AU  - Theodorou GL
FAU - Pagoni, Maria
AU  - Pagoni M
FAU - Galanopoulos, Athanasios
AU  - Galanopoulos A
FAU - Kakagianni, Theodora
AU  - Kakagianni T
FAU - Kouraklis-Symeonidis, Alexandra
AU  - Kouraklis-Symeonidis A
FAU - Matsouka, Panagiota
AU  - Matsouka P
FAU - Maniatis, Alice
AU  - Maniatis A
FAU - Zoumbos, Nicholas C
AU  - Zoumbos NC
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Interleukin-2)
RN  - 0 (Interleukins)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Anemia, Aplastic/*immunology
MH  - Cytoplasm/immunology
MH  - Female
MH  - Humans
MH  - Immunophenotyping
MH  - Interferon-gamma/*metabolism
MH  - Interleukin-10/metabolism
MH  - Interleukin-2/metabolism
MH  - Interleukin-4/metabolism
MH  - Interleukins/*metabolism
MH  - Male
MH  - Middle Aged
MH  - T-Lymphocyte Subsets/immunology
MH  - Th1 Cells/*immunology
MH  - Th2 Cells/*immunology
EDAT- 2003/12/17 05:00
MHDA- 2004/02/03 05:00
CRDT- 2003/12/17 05:00
PHST- 2003/12/17 05:00 [pubmed]
PHST- 2004/02/03 05:00 [medline]
PHST- 2003/12/17 05:00 [entrez]
AID - 4729 [pii]
AID - 10.1046/j.1365-2141.2003.04729.x [doi]
PST - ppublish
SO  - Br J Haematol. 2004 Jan;124(1):97-105. doi: 10.1046/j.1365-2141.2003.04729.x.