PMID- 14675830
OWN - NLM
STAT- MEDLINE
DCOM- 20040224
LR  - 20191108
IS  - 1050-4648 (Print)
IS  - 1050-4648 (Linking)
VI  - 16
IP  - 1
DP  - 2004 Jan
TI  - Cloning and analysis of expression of a gilthead sea bream (Sparus aurata) Mx 
      cDNA.
PG  - 11-24
AB  - In the current work, we have cloned and sequenced the full cDNA for a Mx protein 
      in the gilthead sea bream (Sparus aurata) by RACE PCR. The Mx cDNA of 2182 bp 
      contained an open reading frame of 1857 bp that codes for a protein of 618 aa. 
      Within the coding sequence, characteristic features of Mx proteins were found, 
      such as a tripartite guanosine-5'-triphosphate (GTP)-binding motif (GXXXSGKS/T, 
      DXXG and T/NKXD), the signature of the dynamin family, LPRG(S/K)GIVTR, and a 
      sequence that codes for a leucine zipper at the C-terminal region of the protein. 
      An RT-PCR was optimised to estimate the level of expression of Mx protein in sea 
      bream. Through this method we determined that Mx is constitutively expressed in 
      head kidney, liver, spleen, heart, gills, muscle and brain of healthy sea bream. 
      Intramuscular challenge of sea bream with polyinosinic:polycytidylic acid (Poly 
      I:C) up-regulated Mx expression in liver, head kidney, spleen and muscle. 
      Constitutive expression was also found in isolated head kidney macrophages and 
      blood leukocytes. This expression was significantly up-regulated by addition of 
      Poly I:C. Mx was not constitutively expressed in the sea bream established cell 
      line, SAF-1, but Poly I:C and nodavirus were also capable of inducing Mx 
      expression in this cell line.
FAU - Tafalla, C
AU  - Tafalla C
AD  - Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones 
      Cientificas, Eduardo Cabello 6, Vigo 36208, Spain.
FAU - Aranguren, R
AU  - Aranguren R
FAU - Secombes, C J
AU  - Secombes CJ
FAU - Figueras, A
AU  - Figueras A
FAU - Novoa, B
AU  - Novoa B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Myxovirus Resistance Proteins)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - EC 3.6.5.5 (Dynamins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - DNA Primers
MH  - DNA, Complementary/genetics
MH  - Dynamins/genetics
MH  - GTP-Binding Proteins/*genetics
MH  - *Gene Expression
MH  - Leucine Zippers/genetics
MH  - Molecular Sequence Data
MH  - Myxovirus Resistance Proteins
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sea Bream/*genetics
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
EDAT- 2003/12/17 05:00
MHDA- 2004/02/26 05:00
CRDT- 2003/12/17 05:00
PHST- 2003/12/17 05:00 [pubmed]
PHST- 2004/02/26 05:00 [medline]
PHST- 2003/12/17 05:00 [entrez]
AID - S105046480300010X [pii]
AID - 10.1016/s1050-4648(03)00010-x [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2004 Jan;16(1):11-24. doi: 10.1016/s1050-4648(03)00010-x.