PMID- 14678211
OWN - NLM
STAT- MEDLINE
DCOM- 20040408
LR  - 20191210
IS  - 0105-1873 (Print)
IS  - 0105-1873 (Linking)
VI  - 49
IP  - 3
DP  - 2003 Sep
TI  - Use of the local lymph node assay in the evaluation of the sensitizing potential 
      of pharmaceutical process intermediates.
PG  - 148-54
AB  - The murine local lymph node assay (LLNA) has recently been developed to determine 
      the contact sensitization potential of chemicals. Since its original development, 
      the LLNA results have been the subject of extensive comparisons with guinea pig 
      and human data. The investigations described here were designed to explore the 
      ability of the LLNA to identify accurately, pharmaceutical process intermediates 
      (PIs) known to cause contact allergy in humans. To that end, 16 PIs previously 
      tested in the guinea-pig maximization test (GPMT) were tested in the LLNA. 
      Another PI known to be a contact sensitizer in humans was tested only in the 
      LLNA. Cases of contact sensitization in humans were reported only for PIs that 
      were extreme sensitizers in the GPMT and had low EC3 values (concentration of the 
      test substance required to generate a threefold increase in lymph node cell 
      proliferation) in the LLNA. These data provide additional evidence that the LLNA 
      is able to discriminate skin sensitizers from chemicals that do not possess a 
      significant skin sensitization potential and is thus a useful method for hazard 
      identification. In addition, this method also offers important animal welfare 
      benefits and may also be useful for risk assessment purposes.
FAU - Durand, Genevieve
AU  - Durand G
AD  - MSD-Chibret, Riom, France. genevieve_durand@merck.com
FAU - de Burlet, Georges
AU  - de Burlet G
FAU - Virat, Michel
AU  - Virat M
FAU - Nauman, Bruce D
AU  - Nauman BD
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - Contact Dermatitis
JT  - Contact dermatitis
JID - 7604950
RN  - 0 (Allergens)
RN  - 0 (Pharmaceutical Solutions)
SB  - IM
MH  - Allergens/*adverse effects
MH  - Animals
MH  - Dermatitis, Allergic Contact/diagnosis/*etiology
MH  - Female
MH  - Guinea Pigs
MH  - Humans
MH  - *Local Lymph Node Assay
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Pharmaceutical Solutions/*adverse effects
MH  - Predictive Value of Tests
EDAT- 2003/12/18 05:00
MHDA- 2004/04/09 05:00
CRDT- 2003/12/18 05:00
PHST- 2003/12/18 05:00 [pubmed]
PHST- 2004/04/09 05:00 [medline]
PHST- 2003/12/18 05:00 [entrez]
AID - 197 [pii]
AID - 10.1111/j.0105-1873.2003.00197.x [doi]
PST - ppublish
SO  - Contact Dermatitis. 2003 Sep;49(3):148-54. doi: 10.1111/j.0105-1873.2003.00197.x.